SH2-B Antibody (E-8): sc-393395

SH2-B Antibody (E-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects SH2-B protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SH2-B (E-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SH2-B protein, also known as SH2B1 or PSM, is a 756 amino acid protein that plays a crucial role in the signaling network, particularly in regulating cell shape and movement, which is vital for processes such as cell migration and tissue development. SH2-B protein belongs to the APS family of adapter proteins, characterized by a pleckstrin homology (PH) domain, an SH2 domain, and a tyrosine phosphorylation site, which are essential for signal transduction. SH2-B protein is alternatively spliced to produce three distinct isoforms—SH2-B α, β, and γ—that share a common N-terminal sequence, including the PH domain, SH2 domain, and several proline-rich motifs. These domains enable SH2-B protein to shuttle between the nucleus and cytoplasm, facilitating cellular signaling. SH2-B protein is widely expressed, with highest levels found in skeletal muscle and ovary, and undergoes tyrosine phosphorylation in response to receptor kinase stimulation, further underscoring its importance in cellular communication and response mechanisms.

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SH2-B Antibody (E-8) References:

1. SH2-B is required for nerve growth factor-induced neuronal differentiation.  |  Rui, L., et al. 1999. J Biol Chem. 274: 10590-4. PMID: 10187854
2. Identification of SH2-bbeta as a potent cytoplasmic activator of the tyrosine kinase Janus kinase 2.  |  Rui, L. and Carter-Su, C. 1999. Proc Natl Acad Sci U S A. 96: 7172-7. PMID: 10377387
3. APS, an adapter protein with a PH and SH2 domain, is a substrate for the insulin receptor kinase.  |  Ahmed, Z., et al. 1999. Biochem J. 341 (Pt 3): 665-8. PMID: 10417330
4. SH2-B, a membrane-associated adapter, is phosphorylated on multiple serines/threonines in response to nerve growth factor by kinases within the MEK/ERK cascade.  |  Rui, L., et al. 1999. J Biol Chem. 274: 26485-92. PMID: 10473609
5. Differential binding to and regulation of JAK2 by the SH2 domain and N-terminal region of SH2-bbeta.  |  Rui, L., et al. 2000. Mol Cell Biol. 20: 3168-77. PMID: 10757801
6. Regions of the JAK2 tyrosine kinase required for coupling to the growth hormone receptor.  |  Frank, SJ., et al. 1995. J Biol Chem. 270: 14776-85. PMID: 7540178
7. Identification of SH2-Bbeta as a substrate of the tyrosine kinase JAK2 involved in growth hormone signaling.  |  Rui, L., et al. 1997. Mol Cell Biol. 17: 6633-44. PMID: 9343427
8. Platelet-derived growth factor (PDGF) stimulates the association of SH2-Bbeta with PDGF receptor and phosphorylation of SH2-Bbeta.  |  Rui, L. and Carter-Su, C. 1998. J Biol Chem. 273: 21239-45. PMID: 9694882

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SH2-B Antibody (E-8) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-SH2-B Antibody (E-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SH2-B Antibody (E-8) Specifications

Alternate Product Name: SH2-B

Product Clone Name: E-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 334-422

Epitope Target: SH2-B

Epitope Species: human

Product Reactivity/Detection: SH2-B

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for SH2-B Antibody (E-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin