SH2-B α/β/γ/δ Antibody (46): sc-136065

SH2-B α/β/γ/δ Antibody (46) is a mouse monoclonal IgG1 antibody that detects SH2-B in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). SH2-B is a 756 amino acid protein that plays a crucial role in the signaling network, particularly in the regulation of cell shape and movement, which is vital for various physiological processes including development, immune response, and tissue repair. SH2-B belongs to the APS family of adapter proteins, characterized by a pleckstrin homology (PH) domain, an SH2 domain, and a tyrosine phosphorylation site, which are essential for signal transduction. Alternative splicing produces three distinct isoforms—SH2-B α, β, and γ—that share a common N-terminal sequence, including the PH domain and multiple proline-rich motifs, allowing for diverse regulatory roles in cellular signaling pathways. SH2-B shows highest expression in skeletal muscle and ovary, and shuttles between the nucleus and cytoplasm for regulatory functions. SH2-B undergoes tyrosine phosphorylation in response to receptor kinase stimulation, a modification critical for activity and interaction with other signaling molecules, thereby influencing various cellular responses.

The preferred SH2-B antibody is SH2-B Antibody (E-8): sc-393395.