SH-PTP1 Antibody (D-11) recommended for detection of SH-PTP1 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA

SH-PTP1 Antibody (D-11): sc-7289

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SH-PTP1 Antibody (D-11) is rated 4.74 out of 5 by 19.
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Datasheets

    • Anti-SH-PTP1 Antibody (D-11) is a mouse monoclonal IgG3 (kappa light chain) SH-PTP1 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 576-595 at the C-terminus of SH-PTP1 of human origin (differs from corresponding mouse sequence by a single amino acid)
    • recommended for detection of SH-PTP1 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA; also reactive with additional species, including and canine and porcine
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; phycoerythrin, FITC, Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM
    • blocking peptide, sc-7289 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SH-PTP1 (D-11): sc-7289.

We have problems detecting transfected SHP-1 with this Ab, although we detect the protein with an anti-Myc tag Ab. Can you send me the sequence of the epitope that this Ab recognizes, because there are 4 SHP1 isoforms and they differ in the C-terminus.

Asked by: Dimitar
Thank you for your question. This monoclonal antibody, SH-PTP1 (D-11): sc-7289, is specific for an epitope mapping between amino acids 576-595 at the C-terminus of SH-PTP1 of human origin (Uniprot Accession number: P29350).
Answered by: Technical Support
Date published: 2017-09-13

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-09
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