Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SGTA CRISPR/Cas9 KO Plasmid (m): sc-424626
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SGTA CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SGTA genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SGTA Antibody (6A4): sc-130557
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SGTA CRISPR/Cas9 KO Plasmid (m) | sc-424626 | 20 µg | $397.00 |
Overview
Mouse Sgta encodes small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA), a cytosolic co-chaperone that binds HSP70/HSP90 complexes and recognizes hydrophobic client proteins through its TPR domains. SGTA participates in quality control of nascent and mislocalized proteins, coordinating triage decisions between folding, membrane targeting, and proteasomal degradation pathways. It is implicated in the BAG6/GET-mediated handling of tail-anchored proteins and in regulation of ubiquitin-dependent turnover, linking SGTA to proteostasis and cellular stress responses. Dysregulated protein homeostasis pathways involving SGTA are relevant to mechanistic studies of neurodegeneration, cancer cell stress adaptation, and disorders of membrane protein biogenesis.
SGTA CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sgta gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sgta together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sgta open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SGTA protein expression.
This CRISPR knockout system enables efficient generation of Sgta-deficient cell models for investigation of SGTA signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sgta exon(s) critical for SGTA function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sgta genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SGTA CRISPR/Cas9 KO Plasmid (m) and SGTA CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sgta locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SGTA HDR Plasmid (m) and SGTA HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sgta homology arms to support homology-directed repair at defined Sgta target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.