Sgo1 Antibody (F-8): sc-393993

Sgo1 Antibody (F-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Sgo1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Sgo1 (F-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Sgo1 protein, also known as shugoshin-like 1, consists of 561 amino acids and is predominantly localized in the nucleus and cytoplasm, with highest expression observed in the testis. Sgo1 plays a critical role in ensuring proper chromosome segregation during cell division by localizing to the inner centromere from prophase to metaphase, where Sgo1 prevents premature dissociation of the cohesin complex from centromeres. By inhibiting phosphorylation of the SA-2 subunit of the cohesin complex, Sgo1 ensures that cohesin remains at the centromere until cleaved by Separase during anaphase. This function is vital for accurate attachment of spindle microtubules to the kinetochore, thereby maintaining genomic stability. Additionally, Sgo1 may be involved in the spindle-assembly checkpoint′s tension-sensing mechanism by modulating affinity of Polo-like kinase at the kinetochore. Notably, Sgo1 exists in seven alternatively spliced isoforms, one of which (isoform 3) does not localize to kinetochores at any stage of the cell cycle, highlighting the complexity of regulatory mechanisms.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Sgo1 Antibody (F-8) References:

1. Human Bub1 protects centromeric sister-chromatid cohesion through Shugoshin during mitosis.  |  Tang, Z., et al. 2004. Proc Natl Acad Sci U S A. 101: 18012-7. PMID: 15604152
2. The centromeric protein Sgo1 is required to sense lack of tension on mitotic chromosomes.  |  Indjeian, VB., et al. 2005. Science. 307: 130-3. PMID: 15637284
3. Shugoshin: a centromeric guardian senses tension.  |  Goulding, SE. and Earnshaw, WC. 2005. Bioessays. 27: 588-91. PMID: 15892113
4. A REC8-dependent plant Shugoshin is required for maintenance of centromeric cohesion during meiosis and has no mitotic functions.  |  Hamant, O., et al. 2005. Curr Biol. 15: 948-54. PMID: 15916952
5. The spindle checkpoint: tension versus attachment.  |  Pinsky, BA. and Biggins, S. 2005. Trends Cell Biol. 15: 486-93. PMID: 16084093
6. Regulated separation of sister centromeres depends on the spindle assembly checkpoint but not on the anaphase promoting complex/cyclosome.  |  Giménez-Abián, JF., et al. 2005. Cell Cycle. 4: 1561-75. PMID: 16205119
7. The core centromere and Sgo1 establish a 50-kb cohesin-protected domain around centromeres during meiosis I.  |  Kiburz, BM., et al. 2005. Genes Dev. 19: 3017-30. PMID: 16357219
8. Control of Shugoshin function during fission-yeast meiosis.  |  Vaur, S., et al. 2005. Curr Biol. 15: 2263-70. PMID: 16360688
9. Phosphorylation of human Sgo1 by NEK2A is essential for chromosome congression in mitosis.  |  Fu, G., et al. 2007. Cell Res. 17: 608-18. PMID: 17621308

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Sgo1 Antibody (F-8) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Sgo1 Antibody (F-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Sgo1 Antibody (F-8) Specifications

Alternate Product Name: Sgo1

Product Clone Name: F-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-116

Epitope Target: Sgo1

Epitope Species: human

Product Reactivity/Detection: Sgo1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Sgo1 Antibody (F-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin