SgK223 Antibody (A-6): sc-398164

SgK223 Antibody (A-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects SgK223 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-SgK223 antibody (A-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SgK223, also known as sugen kinase 223, plays a crucial role in cellular signaling pathways, particularly in the regulation of Rho GTPases, which are essential for controlling cytoskeletal dynamics and cell morphology. Specifically, SgK223 interacts with Rnd2, a member of the Rho family, in a GTP-dependent manner, which enhances Rho A activity and promotes cell contraction, thereby influencing neurite outgrowth. This interaction is vital for neuronal development and plasticity, highlighting SgK223′s importance in neurobiology and potential implications in understanding neurodegenerative diseases and neuronal injuries. SgK223 (A-6) monoclonal antibody′s ability to effectively detect SgK223 across multiple species and applications makes this reagent an invaluable tool for researchers studying the intricate mechanisms of cell signaling and development.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SgK223 Antibody (A-6) References:

1. Pragmin, a novel effector of Rnd2 GTPase, stimulates RhoA activity.  |  Tanaka, H., et al. 2006. J Biol Chem. 281: 10355-64. PMID: 16481321
2. Expression of the ras-related ralA, rho12 and rab genes in adult mouse tissues.  |  Olofsson, B., et al. 1988. Oncogene. 3: 231-4. PMID: 2457862
3. Identification of distinct cytoplasmic targets for ras/R-ras and rho regulatory proteins.  |  Garrett, MD., et al. 1989. J Biol Chem. 264: 10-3. PMID: 2491843
4. Scrape-loading of Swiss 3T3 cells with ras protein rapidly activates protein kinase C in the absence of phosphoinositide hydrolysis.  |  Morris, JD., et al. 1989. Oncogene. 4: 27-31. PMID: 2536916
5. The ras superfamily proteins.  |  Chardin, P. 1988. Biochimie. 70: 865-8. PMID: 3145021
6. ras genes.  |  Barbacid, M. 1987. Annu Rev Biochem. 56: 779-827. PMID: 3304147
7. Nucleotide sequence of human rho cDNA clone 12.  |  Yeramian, P., et al. 1987. Nucleic Acids Res. 15: 1869. PMID: 3822842
8. A novel ras-related gene family.  |  Madaule, P. and Axel, R. 1985. Cell. 41: 31-40. PMID: 3888408
9. A new member of the Rho family, Rnd1, promotes disassembly of actin filament structures and loss of cell adhesion.  |  Nobes, CD., et al. 1998. J Cell Biol. 141: 187-97. PMID: 9531558

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SgK223 Antibody (A-6) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-SgK223 Antibody (A-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SgK223 Antibody (A-6) Specifications

Alternate Product Name: SgK223

Product Clone Name: A-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-56

Epitope Target: SgK223

Epitope Species: human

Product Reactivity/Detection: SgK223

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for SgK223 Antibody (A-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin