SFRS16 Antibody (E-7): sc-514890
- SFRS16 Antibody (E-7) is a mouse monoclonal IgG2a κ SFRS16 antibody provided at 200 µg/ml
- raised against amino acids 28-152 mapping near the N-terminus of SFRS16 of human origin
- SFRS16 Antibody (E-7) is recommended for detection of SFRS16 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-SFRS16 Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SFRS16 Antibody (E-7) for WB applications. These reagents are now offered in bundles with SFRS16 Antibody (E-7) (see ordering information below).
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SFRS16 Antibody (E-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects SFRS16 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SFRS16 (E-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SFRS16, also known as splicing factor, arginine/serine-rich 16, plays a crucial role in pre-mRNA splicing regulation, essential for accurate gene expression. Located primarily in the nucleus, SFRS16 recognizes splice sites and modulates alternative splicing, processes vital for generating protein diversity and regulating gene expression in response to cellular signals. SFRS16 shows high expression in the brain, where SFRS16 influences CLK1 mRNA splicing, impacting neuronal function. SFRS16 exists in two alternatively spliced isoforms and undergoes post-translational modifications, including phosphorylation by CLK4, which regulates activity and interactions with other splicing factors. SFRS16 interacts with other serine/arginine-rich splicing factors, such as SC35 and SF2/ASF, highlighting SFRS16′s significance in splicing machinery and potential as a target for studying splicing-related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SFRS16 Antibody (E-7) References:
- Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences. | Schaal, TD. and Maniatis, T. 1999. Mol Cell Biol. 19: 1705-19. PMID: 10022858
- The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. | Cavaloc, Y., et al. 1999. RNA. 5: 468-83. PMID: 10094314
- Regulation of alternative splicing by SRrp86 through coactivation and repression of specific SR proteins. | Barnard, DC., et al. 2002. RNA. 8: 526-33. PMID: 11991645
- Novel SR-rich-related protein clasp specifically interacts with inactivated Clk4 and induces the exon EB inclusion of Clk. | Katsu, R., et al. 2002. J Biol Chem. 277: 44220-8. PMID: 12169693
- Splicing-factor alterations in cancers. | Anczuków, O. and Krainer, AR. 2016. RNA. 22: 1285-301. PMID: 27530828
- Splicing factor gene mutations in hematologic malignancies. | Saez, B., et al. 2017. Blood. 129: 1260-1269. PMID: 27940478
- Splicing factor mutations in the myelodysplastic syndromes: target genes and therapeutic approaches. | Armstrong, RN., et al. 2018. Adv Biol Regul. 67: 13-29. PMID: 28986033
- ERβ agonist alters RNA splicing factor expression and has a longer window of antidepressant effectiveness than estradiol after long-term ovariectomy. | Hou, X., et al. 2019. J Psychiatry Neurosci. 44: 19. PMID: 30565903
- Splicing factor mutant myelodysplastic syndromes: Recent advances. | Pellagatti, A. and Boultwood, J. 2020. Adv Biol Regul. 75: 100655. PMID: 31558432
- Splicing factor SRSF2-centric gene regulation. | Li, K. and Wang, Z. 2021. Int J Biol Sci. 17: 1708-1715. PMID: 33994855
- Specific commitment of different pre-mRNAs to splicing by single SR proteins. | Fu, XD. 1993. Nature. 365: 82-5. PMID: 8361546
- A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm. | Cáceres, JF., et al. 1998. Genes Dev. 12: 55-66. PMID: 9420331
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SFRS16 Antibody (E-7) | sc-514890 | 200 µg/ml | $316.00 | |||
SFRS16 Antibody (E-7): m-IgG Fc BP-HRP Bundle | sc-531240 | 200 µg Ab; 10 µg BP | $354.00 | |||
SFRS16 Antibody (E-7): m-IgGκ BP-HRP Bundle | sc-524872 | 200 µg Ab, 40 µg BP | $354.00 | |||
SFRS16 Antibody (E-7): m-IgG2a BP-HRP Bundle | sc-547737 | 200 µg Ab; 10 µg BP | $354.00 | |||
SFRS16 Antibody (E-7) AC | sc-514890 AC | 500 µg/ml, 25% agarose | $416.00 | |||
SFRS16 Antibody (E-7) HRP | sc-514890 HRP | 200 µg/ml | $316.00 | |||
SFRS16 Antibody (E-7) FITC | sc-514890 FITC | 200 µg/ml | $330.00 | |||
SFRS16 Antibody (E-7) PE | sc-514890 PE | 200 µg/ml | $343.00 | |||
SFRS16 Antibody (E-7) Alexa Fluor® 488 | sc-514890 AF488 | 200 µg/ml | $357.00 | |||
SFRS16 Antibody (E-7) Alexa Fluor® 546 | sc-514890 AF546 | 200 µg/ml | $357.00 | |||
SFRS16 Antibody (E-7) Alexa Fluor® 594 | sc-514890 AF594 | 200 µg/ml | $357.00 | |||
SFRS16 Antibody (E-7) Alexa Fluor® 647 | sc-514890 AF647 | 200 µg/ml | $357.00 | |||
SFRS16 Antibody (E-7) Alexa Fluor® 680 | sc-514890 AF680 | 200 µg/ml | $357.00 | |||
SFRS16 Antibody (E-7) Alexa Fluor® 790 | sc-514890 AF790 | 200 µg/ml | $357.00 |