SF-1 Antibody (A-1): sc-393592

SF-1 Antibody (A-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects SF-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SF-1 Antibody (A-1) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Steroidogenic factor-1 (SF-1), also known as NR5A1, plays a crucial role in regulating genes essential for adrenal and gonadal development, as well as biosynthesis of adrenal and gonadal steroids, anti-Mullerian hormone, and gonadotropins. SF-1 is primarily located in the nucleus of theca/interstitial cells in adult ovary, where SF-1 is vital for proper functioning of steroidogenic pathways. Nuclear localization of SF-1 is significant because SF-1 directly influences gene expression by binding to specific DNA sequences, thereby modulating transcription of target genes involved in hormone production and reproductive development. This regulatory function underscores SF-1′s importance in maintaining hormonal balance and reproductive health, making anti-SF-1 antibody (A-1) an essential tool for researchers studying endocrine function and related disorders.

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SF-1 Antibody (A-1) References:

1. Differential expression of steroidogenic factor-1 and FTF/LRH-1 in the rodent ovary.  |  Falender, AE., et al. 2003. Endocrinology. 144: 3598-610. PMID: 12865342
2. SUMO modification of repression domains modulates function of nuclear receptor 5A1 (steroidogenic factor-1).  |  Chen, WY., et al. 2004. J Biol Chem. 279: 38730-5. PMID: 15192092
3. Of mice and men: The tale of steroidogenic factor-1.  |  Jameson, JL. 2004. J Clin Endocrinol Metab. 89: 5927-9. PMID: 15579738
4. Testicular dysgenesis without adrenal insufficiency in a 46,XY patient with a heterozygous inactive mutation of steroidogenic factor-1.  |  Hasegawa, T., et al. 2004. J Clin Endocrinol Metab. 89: 5930-5. PMID: 15579739
5. The transcription factors steroidogenic factor-1 and SOX9 regulate expression of Vanin-1 during mouse testis development.  |  Wilson, MJ., et al. 2005. J Biol Chem. 280: 5917-23. PMID: 15590666
6. Tissue-specific knockouts of steroidogenic factor 1.  |  Parker, KL. 2004. Endocr Res. 30: 855. PMID: 15666836
7. Crystallographic identification and functional characterization of phospholipids as ligands for the orphan nuclear receptor steroidogenic factor-1.  |  Li, Y., et al. 2005. Mol Cell. 17: 491-502. PMID: 15721253
8. DAX-1 (NR0B1) and steroidogenic factor-1 (SF-1, NR5A1) in human disease.  |  Suntharalingham, JP., et al. 2015. Best Pract Res Clin Endocrinol Metab. 29: 607-19. PMID: 26303087
9. Diagnostic and prognostic utility of SF-1 in adrenal cortical tumours.  |  Maity, P., et al. 2022. Indian J Pathol Microbiol. 65: 814-820. PMID: 36308186
10. Steroidogenic factor 1 (SF-1; Nr5a1) regulates the formation of the ovarian reserve.  |  Hughes, CHK., et al. 2023. Proc Natl Acad Sci U S A. 120: e2220849120. PMID: 37494420
11. Pituitary neuroendocrine tumors with PIT1/SF1 co-expression show distinct clinicopathological and molecular features.  |  Dottermusch, M., et al. 2024. Acta Neuropathol. 147: 16. PMID: 38228887
12. Cloning and characterization of a novel human hepatocyte transcription factor, hB1F, which binds and activates enhancer II of hepatitis B virus.  |  Li, M., et al. 1998. J Biol Chem. 273: 29022-31. PMID: 9786908

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SF-1 Antibody (A-1) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-SF-1 Antibody (A-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SF-1 Antibody (A-1) Specifications

Alternate Product Name: NR5A1

Product Clone Name: A-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 228-263

Epitope Target: SF-1

Epitope Species: human

Product Reactivity/Detection: SF-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for SF-1 Antibody (A-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent