SETD4 Antibody (F-3) recommended for detection of SETD4 of mouse, rat and human origin by WB, IP, IF and ELISA

SETD4 Antibody (F-3): sc-514060

SETD4 Antibody (F-3) is rated 5.0 out of 5 by 6.
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Datasheets

    • Anti-SETD4 Antibody (F-3) is a mouse monoclonal IgG1 (kappa light chain) SETD4 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 24-51 near the N-terminus of SETD4 of human origin
    • Anti-SETD4 Antibody (F-3) is recommended for detection of SETD4 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-SETD4 Antibody (F-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-514060 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SETD4 (F-3): sc-514060.
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What application is the blocking peptide sc-514060 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from IF detection SETD4 was highly expressed in samples from mice arthritis model, and this antibody gave a good response to the experimental results, we used it for IF (1:300),very specific.
Date published: 2017-09-09
Rated 5 out of 5 by from IF detection We used SETD4 antibody to detect the localization of SETD4 in different cells by immunofluorescence ,We got the target staining at right position for different cell lines,very nice.
Date published: 2017-08-26
Rated 5 out of 5 by from Specific staining This antibody was used for staining of HepG2 cells . Result: Specific staining of the membrane, very low background at 1:200 concentration,also the staining is specific.
Date published: 2017-08-18
Rated 5 out of 5 by from expected result We were looking to detect differences in SETD4 expression in treated mouse brain lysates. it worked very well with and exceeding my expectations ,very strong singal and clean specific band at 1:800 dilution.
Date published: 2017-07-29
Rated 5 out of 5 by from Good Western Blot data of SETD4 expression Good Western Blot data of SETD4 expression in rat cerebellum and human testis tissue extracts and HL-60, K-562, HEK293 and Hep G2 whole cell lysates. -SCBT QC
Date published: 2015-03-14
Rated 5 out of 5 by from Great Western Blot data of SETD4 expression Great Western Blot data of SETD4 expression in human tonsil tissue extract. -SCBT QC
Date published: 2014-05-09
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