Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Septin 8 CRISPR/Cas9 KO Plasmid (m): sc-422891
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Septin 8 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Septin 8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Septin 8 Antibody (C-5): sc-390074
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Septin 8 CRISPR/Cas9 KO Plasmid (m) | sc-422891 | 20 µg | $397.00 |
Overview
Sept8 encodes septin 8, a GTP-binding cytoskeletal protein that assembles into hetero-oligomeric septin filaments and ring-like structures that scaffold membranes and organize the actin–microtubule interface. Septin 8 contributes to cytokinesis, cell polarity, and vesicle trafficking, and it helps compartmentalize signaling by forming diffusion barriers at specialized membrane domains. In neurons, septin complexes containing septin 8 are linked to neurite morphogenesis and synaptic organization, supporting studies of development and circuit maintenance in mouse models. Dysregulated septin network dynamics have been associated with altered cell division and cytoskeletal remodeling observed in cancer and neurodegenerative research contexts, making Sept8 a useful target for mechanistic pathway interrogation.
Septin 8 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sept8 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sept8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sept8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Septin 8 protein expression.
This CRISPR knockout system enables efficient generation of Sept8-deficient cell models for investigation of Septin 8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sept8 exon(s) critical for Septin 8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sept8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Septin 8 CRISPR/Cas9 KO Plasmid (m) and Septin 8 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sept8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Septin 8 HDR Plasmid (m) and Septin 8 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sept8 homology arms to support homology-directed repair at defined Sept8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.