Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SENP8 CRISPR/Cas9 KO Plasmid (h): sc-405158
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SENP8 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SENP8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SENP8 Antibody (E-11): sc-271498
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SENP8 CRISPR/Cas9 KO Plasmid (h) | sc-405158 | 20 µg | $397.00 |
Overview
SENP8 encodes a cysteine protease that functions as a deneddylase with specificity toward NEDD8-conjugated substrates and contributes to maintaining NEDD8 pathway homeostasis. By modulating the neddylation status of cullin-RING ligases, SENP8 influences ubiquitin-dependent protein turnover, cell cycle progression, and stress-responsive signaling. SENP8 activity is also linked to regulation of DCN1-dependent cullin neddylation dynamics and overall proteostasis control. Dysregulation of NEDD8 cycling and cullin ligase activity has been associated with oncogenic phenotypes and neurodevelopmental or neurodegenerative processes, making SENP8 a relevant node for mechanistic studies.
SENP8 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SENP8 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SENP8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SENP8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SENP8 protein expression.
This CRISPR knockout system enables efficient generation of SENP8-deficient cell models for investigation of SENP8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SENP8 exon(s) critical for SENP8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SENP8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SENP8 CRISPR/Cas9 KO Plasmid (h) and SENP8 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SENP8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SENP8 HDR Plasmid (h) and SENP8 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SENP8 homology arms to support homology-directed repair at defined SENP8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.