selenocysteine lyase Antibody (B-8): sc-374391
- selenocysteine lyase Antibody (B-8) is a mouse monoclonal IgG1 κ selenocysteine lyase antibody provided at 200 µg/ml
- raised against amino acids 291-368 mapping within an internal region of selenocysteine lyase of human origin
- selenocysteine lyase Antibody (B-8) is recommended for detection of selenocysteine lyase of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-selenocysteine lyase Antibody (B-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for selenocysteine lyase Antibody (B-8) for WB and IHC(P) applications. These reagents are now offered in bundles with selenocysteine lyase Antibody (B-8) (see ordering information below).
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Selenocysteine lyase Antibody (B-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects selenocysteine lyase of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-selenocysteine lyase antibody (B-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Selenocysteine lyase (SCL) plays a crucial role in selenium metabolism by catalyzing the conversion of L-selenocysteine to L-alanine and elemental selenium, a process vital for maintaining selenium homeostasis in the body. This enzymatic reaction requires pyridoxal 5′-phosphate as a cofactor and predominantly occurs in tissues such as the liver, kidney, heart, adrenal glands, and muscle. SCL functions as a selenium delivery protein to selenophosphate synthetase, facilitating the biosynthesis of selenoproteins, which are essential for various biological processes, including antioxidant defense and thyroid hormone metabolism. The regulation of SCL by pyridoxal 5′-phosphate mirrors regulatory mechanisms observed in other enzymes, such as aspartate beta-decarboxylase and arginine racemase, highlighting its significance in metabolic pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
selenocysteine lyase Antibody (B-8) References:
- cDNA cloning, purification, and characterization of mouse liver selenocysteine lyase. Candidate for selenium delivery protein in selenoprotein synthesis. | Mihara, H., et al. 2000. J Biol Chem. 275: 6195-200. PMID: 10692412
- Kinetic and mutational studies of three NifS homologs from Escherichia coli: mechanistic difference between L-cysteine desulfurase and L-selenocysteine lyase reactions. | Mihara, H., et al. 2000. J Biochem. 127: 559-67. PMID: 10739946
- Selenocysteine lyase from mouse liver. | Mihara, H. and Esaki, N. 2002. Methods Enzymol. 347: 198-203. PMID: 11898407
- Enhanced selenium tolerance and accumulation in transgenic Arabidopsis expressing a mouse selenocysteine lyase. | Pilon, M., et al. 2003. Plant Physiol. 131: 1250-7. PMID: 12644675
- Characterization of selenocysteine lyase in human tissues and its relationship to tissue selenium concentrations. | Daher, R. and Van Lente, F. 1992. J Trace Elem Electrolytes Health Dis. 6: 189-94. PMID: 1483038
- Methanococcus vannielii selenium metabolism: purification and N-terminal amino acid sequences of a novel selenium-binding protein and selenocysteine lyase. | Stadtman, T. 2004. IUBMB Life. 56: 427-31. PMID: 15545220
- Mechanism of reactions catalyzed by selenocysteine beta-lyase. | Esaki, N., et al. 1985. Arch Biochem Biophys. 238: 418-23. PMID: 3158280
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
selenocysteine lyase Antibody (B-8) | sc-374391 | 200 µg/ml | $322.00 | |||
selenocysteine lyase Antibody (B-8): m-IgG Fc BP-HRP Bundle | sc-527458 | 200 µg Ab; 10 µg BP | $361.00 | |||
selenocysteine lyase Antibody (B-8): m-IgG1 BP-HRP Bundle | sc-532831 | 200 µg Ab; 20 µg BP | $361.00 | |||
selenocysteine lyase Antibody (B-8) AC | sc-374391 AC | 500 µg/ml, 25% agarose | $424.00 | |||
selenocysteine lyase Antibody (B-8) HRP | sc-374391 HRP | 200 µg/ml | $322.00 | |||
selenocysteine lyase Antibody (B-8) FITC | sc-374391 FITC | 200 µg/ml | $336.00 | |||
selenocysteine lyase Antibody (B-8) PE | sc-374391 PE | 200 µg/ml | $349.00 | |||
selenocysteine lyase Antibody (B-8) Alexa Fluor® 488 | sc-374391 AF488 | 200 µg/ml | $364.00 | |||
selenocysteine lyase Antibody (B-8) Alexa Fluor® 546 | sc-374391 AF546 | 200 µg/ml | $364.00 | |||
selenocysteine lyase Antibody (B-8) Alexa Fluor® 594 | sc-374391 AF594 | 200 µg/ml | $364.00 | |||
selenocysteine lyase Antibody (B-8) Alexa Fluor® 647 | sc-374391 AF647 | 200 µg/ml | $364.00 | |||
selenocysteine lyase Antibody (B-8) Alexa Fluor® 680 | sc-374391 AF680 | 200 µg/ml | $364.00 | |||
selenocysteine lyase Antibody (B-8) Alexa Fluor® 790 | sc-374391 AF790 | 200 µg/ml | $364.00 |