Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Sec8 CRISPR/Cas9 KO Plasmid (m): sc-422866
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sec8 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Sec8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Sec8 Antibody (B-11): sc-514215
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sec8 CRISPR/Cas9 KO Plasmid (m) | sc-422866 | 20 µg | $397.00 |
Overview
Exoc4 encodes Sec8, an essential subunit of the octameric exocyst complex that mediates tethering of secretory vesicles to defined plasma membrane sites prior to SNARE-dependent fusion. In mouse cells, Sec8 helps coordinate polarized exocytosis and membrane trafficking that support processes such as epithelial polarity, neurite outgrowth, cytokinesis, and receptor recycling, integrating signals from small GTPases including Ral, Rab, and Cdc42. Exocyst function is closely linked to cytoskeletal remodeling and directed cell migration, making Sec8 a useful node for studying pathways that govern tissue organization and intracellular transport fidelity. Dysregulation of exocyst-dependent trafficking has been associated with altered cell polarity and aberrant signaling dynamics relevant to developmental and neurobiology research.
Sec8 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Exoc4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Exoc4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Exoc4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Sec8 protein expression.
This CRISPR knockout system enables efficient generation of Exoc4-deficient cell models for investigation of Sec8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Exoc4 exon(s) critical for Sec8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Exoc4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Sec8 CRISPR/Cas9 KO Plasmid (m) and Sec8 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Exoc4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Sec8 HDR Plasmid (m) and Sec8 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Exoc4 homology arms to support homology-directed repair at defined Exoc4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.