Sec8 Antibody (LB-2): sc-100734
- Sec8 Antibody (LB-2) is a mouse monoclonal IgG2a κ, cited in 1 publications, provided at 100 µg/ml
- raised against recombinant Sec8 of human origin
- recommended for detection of Sec8 of mouse, rat and human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Sec8 Antibody (LB-2). This work is in progress.
Sec8 Antibody (LB-2) is a mouse monoclonal IgG2a antibody that detects Sec8 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Sec8, also known as EXOC4, REC8, or SEC8L1, is a critical component of the exocyst complex, which plays a vital role in exocytosis, a process essential for membrane trafficking, hormone and neurotransmitter secretion, and the incorporation of membrane proteins and lipids into the plasma membrane. The exocyst complex, composed of eight protein subunits including Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, and Exo84, is crucial for targeting exocytic vesicles to specific docking sites on the plasma membrane, thereby facilitating effective cell-cell communication, cell growth, and maintaining cell polarity. Notably, Sec8 is involved in modulating microtubule dynamics, as Sec8 has been shown to inhibit tubulin polymerization in vitro, highlighting Sec8′s importance in the regulation of cytoskeletal elements that underlie exocytosis. Human Sec8 is located on chromosome 7q33, and Sec8′s proper functioning is essential for various cellular processes, making anti-Sec8 antibody (LB-2) a valuable tool for researchers studying exocytosis and related cellular mechanisms.
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Sec8 Antibody (LB-2) References:
- Exocyst complex subunit sec8 binds to postsynaptic density protein-95 (PSD-95): a novel interaction regulated by cypin (cytosolic PSD-95 interactor). | Riefler, GM., et al. 2003. Biochem J. 373: 49-55. PMID: 12675619
- A role for Sec8 in oligodendrocyte morphological differentiation. | Anitei, M., et al. 2006. J Cell Sci. 119: 807-18. PMID: 16478790
- Knockdown of Sec8 promotes cell-cycle arrest at G1/S phase by inducing p21 via control of FOXO proteins. | Tanaka, T. and Iino, M. 2014. FEBS J. 281: 1068-84. PMID: 24299491
- Knockdown of Sec8 enhances the binding affinity of c-Jun N-terminal kinase (JNK)-interacting protein 4 for mitogen-activated protein kinase kinase 4 (MKK4) and suppresses the phosphorylation of MKK4, p38, and JNK, thereby inhibiting apoptosis. | Tanaka, T., et al. 2014. FEBS J. 281: 5237-50. PMID: 25244576
- Sec8 regulates cytokeratin8 phosphorylation and cell migration by controlling the ERK and p38 MAPK signalling pathways. | Tanaka, T. and Iino, M. 2015. Cell Signal. 27: 1110-9. PMID: 25725287
- CREG1 Interacts with Sec8 to Promote Cardiomyogenic Differentiation and Cell-Cell Adhesion. | Liu, J., et al. 2016. Stem Cells. 34: 2648-2660. PMID: 27334848
- Sec8 modulates TGF-β induced EMT by controlling N-cadherin via regulation of Smad3/4. | Tanaka, T., et al. 2017. Cell Signal. 29: 115-126. PMID: 27769780
- LRRK2 Modulates the Exocyst Complex Assembly by Interacting with Sec8. | Fais, M., et al. 2021. Cells. 10: PMID: 33498474
- Sec8 specifically interacts with the PDZ2 domain of synapse associated protein 102 (SAP102). | Korbula, K., et al. 2023. Front Cell Dev Biol. 11: 1254611. PMID: 37849738
- Sec6, Sec8, and Sec15 are components of a multisubunit complex which localizes to small bud tips in Saccharomyces cerevisiae. | TerBush, DR. and Novick, P. 1995. J Cell Biol. 130: 299-312. PMID: 7615633
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sec8 Antibody (LB-2) | sc-100734 | 100 µg/ml | $333.00 |