Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Sec61β CRISPR/Cas9 KO Plasmid (m): sc-425892
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sec61β CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Sec61β genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Sec61β Antibody (E-6): sc-393633
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sec61β CRISPR/Cas9 KO Plasmid (m) | sc-425892 | 20 µg | $397.00 |
Overview
SEC61B encodes the beta subunit of the Sec61 translocon, a core component of the endoplasmic reticulum (ER) membrane channel that mediates co-translational protein import and integration of nascent polypeptides into the secretory pathway. In mouse cells, Sec61β supports ER protein biogenesis, coordinates with ribosome docking, and contributes to ER proteostasis processes linked to folding and ER-associated degradation (ERAD). Altered translocon function can perturb secretion, membrane protein homeostasis, and unfolded protein response signaling, connecting SEC61 complex activity to cellular stress adaptation. Because secretory pathway integrity underlies immune signaling, metabolism, and neuronal function, Sec61b is frequently studied in the context of proteostasis imbalance and stress-related disease mechanisms.
Sec61β CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sec61b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sec61b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sec61b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Sec61β protein expression.
This CRISPR knockout system enables efficient generation of Sec61b-deficient cell models for investigation of Sec61β signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sec61b exon(s) critical for Sec61β function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sec61b genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Sec61β CRISPR/Cas9 KO Plasmid (m) and Sec61β CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sec61b locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Sec61β HDR Plasmid (m) and Sec61β HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sec61b homology arms to support homology-directed repair at defined Sec61b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.