Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SEC13 CRISPR/Cas9 KO Plasmid (m): sc-431017
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SEC13 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SEC13 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SEC13 Antibody (F-6): sc-514308
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SEC13 CRISPR/Cas9 KO Plasmid (m) | sc-431017 | 20 µg | $397.00 |
Overview
Sec13 encodes SEC13, a core component of the COPII coat complex that drives cargo selection and vesicle budding from endoplasmic reticulum exit sites to support ER-to-Golgi trafficking. Beyond secretory pathway function, SEC13 participates in nuclear pore complex architecture and contributes to regulation of cellular homeostasis through membrane trafficking–dependent control of protein localization and turnover. Disruption of SEC13-dependent transport can perturb proteostasis, stress signaling, and cell cycle coordination, making Sec13 a useful node for studying pathways that intersect secretion, membrane dynamics, and nuclear-cytoplasmic exchange. In mouse systems, Sec13 loss-of-function models are relevant for probing how trafficking and pore-associated processes influence development and disease-associated cellular phenotypes.
SEC13 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sec13 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sec13 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sec13 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SEC13 protein expression.
This CRISPR knockout system enables efficient generation of Sec13-deficient cell models for investigation of SEC13 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sec13 exon(s) critical for SEC13 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sec13 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SEC13 CRISPR/Cas9 KO Plasmid (m) and SEC13 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sec13 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SEC13 HDR Plasmid (m) and SEC13 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sec13 homology arms to support homology-directed repair at defined Sec13 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.