Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SDHC CRISPR/Cas9 KO Plasmid (h): sc-405559
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SDHC CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SDHC genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SDHC Antibody (C-2): sc-515102
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SDHC CRISPR/Cas9 KO Plasmid (h) | sc-405559 | 20 µg | $397.00 |
Overview
SDHC encodes the succinate dehydrogenase complex subunit C, an integral membrane component of mitochondrial Complex II that anchors the enzyme to the inner mitochondrial membrane and supports electron transfer from succinate to ubiquinone. Through its role at the intersection of the tricarboxylic acid (TCA) cycle and oxidative phosphorylation, SDHC contributes to respiratory chain function, mitochondrial redox balance, and cellular energy metabolism. Disruption of SDH complex integrity can alter succinate handling and downstream signaling pathways linked to metabolic rewiring and stress responses. SDHC dysfunction has been associated with mitochondrial disease mechanisms and tumor-predisposition biology, making it relevant for studying metabolism-driven phenotypes and mitochondrial quality control.
SDHC CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SDHC gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SDHC together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SDHC open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SDHC protein expression.
This CRISPR knockout system enables efficient generation of SDHC-deficient cell models for investigation of SDHC signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SDHC exon(s) critical for SDHC function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SDHC genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SDHC CRISPR/Cas9 KO Plasmid (h) and SDHC CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SDHC locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SDHC HDR Plasmid (h) and SDHC HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SDHC homology arms to support homology-directed repair at defined SDHC target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.