SDHB Antibody (G-10): sc-271548

SDHB Antibody (G-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects SDHB protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-SDHB antibody (G-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SDHB plays a crucial role in the mitochondrial respiratory chain, specifically as part of the succinate dehydrogenase complex, which is essential for aerobic respiration. This complex catalyzes the oxidation of succinate to fumarate, a key step in the tricarboxylic acid cycle, linking the metabolic pathways of the Krebs cycle and oxidative phosphorylation. Proper functioning of SDHB is vital for energy production in cells, and mutations in SDHB can lead to significant mitochondrial dysfunction, resulting in various hereditary disorders. For instance, inactivating mutations in SDHB are associated with inherited cases of pheochromocytoma, a type of tumor that can affect the adrenal glands, while mutations in other subunits of the complex can lead to conditions such as Leigh syndrome and juvenile encephalopathy. Understanding SDHB and its interactions within the succinate dehydrogenase complex is essential for elucidating the mechanisms behind these disorders and developing potential therapeutic strategies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SDHB Antibody (G-10) References:

1. Gene mutations in the succinate dehydrogenase subunit SDHB cause susceptibility to familial pheochromocytoma and to familial paraganglioma.  |  Astuti, D., et al. 2001. Am J Hum Genet. 69: 49-54. PMID: 11404820
2. Novel succinate dehydrogenase subunit B (SDHB) mutations in familial phaeochromocytomas and paragangliomas, but an absence of somatic SDHB mutations in sporadic phaeochromocytomas.  |  Benn, DE., et al. 2003. Oncogene. 22: 1358-64. PMID: 12618761
3. Molecular genetic analysis of FIH-1, FH, and SDHB candidate tumour suppressor genes in renal cell carcinoma.  |  Morris, MR., et al. 2004. J Clin Pathol. 57: 706-11. PMID: 15220362
4. Malignant pheochromocytoma associated with germline mutation of the SDHB gene.  |  Allibhai, Z., et al. 2004. J Urol. 172: 1409-10. PMID: 15371856
5. Loss of succinate dehydrogenase subunit B (SDHB) as a prognostic factor in advanced ileal well-differentiated neuroendocrine tumors.  |  Milione, M., et al. 2017. Endocrine. 57: 512-517. PMID: 27905048
6. Tumour risks and genotype-phenotype correlations associated with germline variants in succinate dehydrogenase subunit genes SDHB, SDHC and SDHD.  |  Andrews, KA., et al. 2018. J Med Genet. 55: 384-394. PMID: 29386252
7. Loss of SDHB Promotes Dysregulated Iron Homeostasis, Oxidative Stress, and Sensitivity to Ascorbate.  |  Goncalves, J., et al. 2021. Cancer Res. 81: 3480-3494. PMID: 34127497
8. Quantifying evidence toward pathogenicity for rare phenotypes: The case of succinate dehydrogenase genes, SDHB and SDHD.  |  Garrett, A., et al. 2022. Genet Med. 24: 41-50. PMID: 34906457
9. Research progress on the pathogenesis of the SDHB mutation and related diseases.  |  Liu, C., et al. 2023. Biomed Pharmacother. 167: 115500. PMID: 37734265
10. Management of phaeochromocytoma and paraganglioma in patients with germline SDHB pathogenic variants: an international expert Consensus statement.  |  Taïeb, D., et al. 2024. Nat Rev Endocrinol. 20: 168-184. PMID: 38097671
11. Mutation of a nuclear succinate dehydrogenase gene results in mitochondrial respiratory chain deficiency.  |  Bourgeron, T., et al. 1995. Nat Genet. 11: 144-9. PMID: 7550341
12. Human complex II (succinate-ubiquinone oxidoreductase): cDNA cloning of the flavoprotein (Fp) subunit of liver mitochondria.  |  Hirawake, H., et al. 1994. J Biochem. 116: 221-7. PMID: 7798181

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SDHB Antibody (G-10) has 21 citations in a variety of scientific publications.

Primary antibodies like Anti-SDHB Antibody (G-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SDHB Antibody (G-10) Specifications

Alternate Product Name: Succinate Dehydrogenase B

Product Clone Name: G-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-280

Epitope Target: succinate dehydrogenase iron-sulfur protein

Epitope Species: human

Product Reactivity/Detection: precursor and mature SDHB

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 21 citations in various scientific publications

Available Conjugates/Forms for SDHB Antibody (G-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin