SCP-3 Antibody (D-1): sc-74569

SCP-3 Antibody (D-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects SCP-3 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-SCP-3 antibody (D-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SCP-3 (D-1) monoclonal antibody recognizes a protein that plays a crucial role in synaptonemal complex formation, essential for proper chromosome pairing and recombination during meiosis. These complexes facilitate accurate chromosome segregation, ensuring genetic diversity and stability in gametes. SCP-3 monoclonal antibody (D-1) detects expression specifically in testicular meiotic prophase cells and primordial germ cells, with presence first noted in leptotene-stage spermatocytes and diminishing in late meiotic cells. Anti-SCP-3 antibody (D-1) targets a protein featuring a C-terminal coiled-coil domain vital for promoting homotypic interactions, critical for synaptonemal complex structural integrity. Understanding SCP-3 function and dynamics helps elucidate meiosis mechanisms and potential implications for fertility and genetic disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SCP-3 Antibody (D-1) References:

1. A meiotic chromosomal core consisting of cohesin complex proteins recruits DNA recombination proteins and promotes synapsis in the absence of an axial element in mammalian meiotic cells.  |  Pelttari, J., et al. 2001. Mol Cell Biol. 21: 5667-77. PMID: 11463847
2. Mammalian STAG3 is a cohesin specific to sister chromatid arms in meiosis I.  |  Prieto, I., et al. 2001. Nat Cell Biol. 3: 761-6. PMID: 11483963
3. Centromere and telomere redistribution precedes homologue pairing and terminal synapsis initiation during prophase I of cattle spermatogenesis.  |  Pfeifer, C., et al. 2001. Cytogenet Cell Genet. 93: 304-14. PMID: 11528130
4. Female germ cell aneuploidy and embryo death in mice lacking the meiosis-specific protein SCP3.  |  Yuan, L., et al. 2002. Science. 296: 1115-8. PMID: 12004129
5. Testicular expression of synaptonemal complex protein 3 (SYCP3) messenger ribonucleic acid in 110 patients with nonobstructive azoospermia.  |  Aarabi, M., et al. 2006. Fertil Steril. 86: 325-31. PMID: 16824523
6. Investigation of mutations in the synaptonemal complex protein 3 (SYCP3) gene among azoospermic infertile male patients in the Turkish population.  |  Gurkan, H., et al. 2013. Andrologia. 45: 92-100. PMID: 22670862
7. Fission yeast Scp3 potentially maintains microtubule orientation through bundling.  |  Ozaki, K., et al. 2015. PLoS One. 10: e0120109. PMID: 25767875
8. Synaptonemal Complex Protein 3 Transcript Analysis in Breast Cancer.  |  Mobasheri, MB., et al. 2016. Iran J Public Health. 45: 1618-1624. PMID: 28053928
9. Targeting Cyclin D-CDK4/6 Sensitizes Immune-Refractory Cancer by Blocking the SCP3-NANOG Axis.  |  Oh, SJ., et al. 2018. Cancer Res. 78: 2638-2653. PMID: 29437706
10. SCP2: a major protein component of the axial elements of synaptonemal complexes of the rat.  |  Offenberg, HH., et al. 1998. Nucleic Acids Res. 26: 2572-9. PMID: 9592139
11. Meiotic activation of rat pachytene spermatocytes with okadaic acid: the behaviour of synaptonemal complex components SYN1/SCP1 and COR1/SCP3.  |  Tarsounas, M., et al. 1999. J Cell Sci. 112 (Pt 4): 423-34. PMID: 9914155
12. Localization of SCP2 and SCP3 protein molecules within synaptonemal complexes of the rat.  |  Schalk, JA., et al. 1998. Chromosoma. 107: 540-8. PMID: 9933407

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SCP-3 Antibody (D-1) has 154 citations in a variety of scientific publications.

Primary antibodies like Anti-SCP-3 Antibody (D-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SCP-3 Antibody (D-1) Specifications

Alternate Product Name: SYCP3

Product Clone Name: D-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-254

Epitope Target: SCP-3

Epitope Species: mouse

Product Reactivity/Detection: SCP-3

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 154 citations in various scientific publications

Available Conjugates/Forms for SCP-3 Antibody (D-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin