SCML2 Antibody (G-1): sc-271228
- SCML2 Antibody (G-1) is a mouse monoclonal IgG2b κ, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 563-655 mapping near the C-terminus of SCML2 of human origin
- recommended for detection of SCML2 of human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for SCML2 Antibody (G-1) for WB and IHC(P) applications. This reagent is now offered in a bundle with SCML2 Antibody (G-1) (see ordering information below).
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SCML2 Antibody (G-1) is a mouse monoclonal IgG2b antibody that detects SCML2 in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). SCML2, a human homolog of the Drosophila Polycomb protein SCM, plays a crucial role in transcriptional repression and is essential for regulating gene expression during development. SCML2 is predominantly expressed in the placenta, testis, and thymus, highlighting SCML2′s importance in reproductive and immune functions. Notably, SCML2 undergoes post-translational modifications, such as phosphorylation by ATR or ATM in response to DNA damage, which can influence SCML2′s activity and stability. Alternative splicing of SCML2 results in two isoforms, allowing for functional diversity and adaptability in various cellular contexts. Anti-SCML2 antibody (G-1) serves as an invaluable tool for researchers studying gene regulation, developmental biology, and cellular response to DNA damage.
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SCML2 Antibody (G-1) References:
- Identification of SCML2, a second human gene homologous to the Drosophila sex comb on midleg (Scm): A new gene cluster on Xp22. | Montini, E., et al. 1999. Genomics. 58: 65-72. PMID: 10331946
- The malignant brain tumor repeats of human SCML2 bind to peptides containing monomethylated lysine. | Santiveri, CM., et al. 2008. J Mol Biol. 382: 1107-12. PMID: 18706910
- Polycomb protein SCML2 regulates the cell cycle by binding and modulating CDK/CYCLIN/p21 complexes. | Lecona, E., et al. 2013. PLoS Biol. 11: e1001737. PMID: 24358021
- Solution NMR structure of the DNA-binding domain from Scml2 (sex comb on midleg-like 2). | Bezsonova, I. 2014. J Biol Chem. 289: 15739-49. PMID: 24727478
- SCML2 establishes the male germline epigenome through regulation of histone H2A ubiquitination. | Hasegawa, K., et al. 2015. Dev Cell. 32: 574-88. PMID: 25703348
- Polycomb protein SCML2 facilitates H3K27me3 to establish bivalent domains in the male germline. | Maezawa, S., et al. 2018. Proc Natl Acad Sci U S A. 115: 4957-4962. PMID: 29686098
- SCML2 promotes heterochromatin organization in late spermatogenesis. | Maezawa, S., et al. 2018. J Cell Sci. 131: PMID: 30097555
- A rapidly evolved domain, the SCML2 DNA-binding repeats, contributes to chromatin binding of mouse SCML2†. | Maezawa, S., et al. 2019. Biol Reprod. 100: 409-419. PMID: 30137219
- Sex Comb on Midleg Like-2 Accelerates Hepatocellular Carcinoma Cell Proliferation and Metastasis by Activating Wnt/β-Catenin/EMT Signaling. | Du, L., et al. 2021. Yonsei Med J. 62: 1073-1082. PMID: 34816637
- Discordant interactions between YAP1 and polycomb group protein SCML2 determine cell fate. | Boston, AM., et al. 2023. iScience. 26: 107964. PMID: 37810219
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SCML2 Antibody (G-1) | sc-271228 | 200 µg/ml | $322.00 | |||
SCML2 Antibody (G-1): m-IgG Fc BP-HRP Bundle | sc-540216 | 200 µg Ab; 10 µg BP | $361.00 |