Sclerostin Antibody (C-11): sc-518161
- Sclerostin Antibody (C-11) is a mouse monoclonal IgM κ, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 31-52 near the N-terminus of Sclerostin of human origin
- recommended for detection of Sclerostin of human origin by WB, IP, IF and ELISA
- Anti-Sclerostin Antibody (C-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for Sclerostin Antibody (C-11) for WB applications. This reagent is now offered in a bundle with Sclerostin Antibody (C-11) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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SEE ALSO...
Sclerostin Antibody (C-11) is a mouse monoclonal IgM kappa antibody that detects Sclerostin of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Sclerostin antibody (C-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Sclerostin, encoded by the SOST gene, plays a crucial role in bone homeostasis by inhibiting osteoblast activity, which is essential for regulating bone formation and resorption. This regulation is particularly important in conditions such as sclerosteosis and Van Buchem disease, where mutations in the SOST gene lead to excessive bone growth and associated complications, including nerve entrapment and facial distortion. Sclerostin is primarily secreted by osteocytes and is typically expressed at low levels; however, expression can increase in response to mechanical loading or certain hormonal signals, highlighting Sclerostin′s role in adapting bone density to physiological demands. Anti-Sclerostin antibody (C-11) effectively detects Sclerostin across various applications, making this reagent a valuable tool for researchers studying bone metabolism and related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Sclerostin Antibody (C-11) References:
- Sclerostin is a novel secreted osteoclast-derived bone morphogenetic protein antagonist with unique ligand specificity. | Kusu, N., et al. 2003. J Biol Chem. 278: 24113-7. PMID: 12702725
- Sclerostin promotes the apoptosis of human osteoblastic cells: a novel regulation of bone formation. | Sutherland, MK., et al. 2004. Bone. 35: 828-35. PMID: 15454089
- Sclerostin inhibition of Wnt-3a-induced C3H10T1/2 cell differentiation is indirect and mediated by bone morphogenetic proteins. | Winkler, DG., et al. 2005. J Biol Chem. 280: 2498-502. PMID: 15545262
- Sclerostin binds to LRP5/6 and antagonizes canonical Wnt signaling. | Li, X., et al. 2005. J Biol Chem. 280: 19883-7. PMID: 15778503
- SOST/sclerostin, an osteocyte-derived negative regulator of bone formation. | van Bezooijen, RL., et al. 2005. Cytokine Growth Factor Rev. 16: 319-27. PMID: 15869900
- Sclerostin is a delayed secreted product of osteocytes that inhibits bone formation. | Poole, KE., et al. 2005. FASEB J. 19: 1842-4. PMID: 16123173
- Bone morphogenetic proteins and their antagonists: the sclerostin paradigm. | van Bezooijen, RL., et al. 2005. J Endocrinol Invest. 28: 15-7. PMID: 16323824
- The Role of Sclerostin in Bone and Ectopic Calcification. | Maré, A., et al. 2020. Int J Mol Sci. 21: PMID: 32366042
- Role of Wnt signaling and sclerostin in bone and as therapeutic targets in skeletal disorders. | Marini, F., et al. 2023. Osteoporos Int. 34: 213-238. PMID: 35982318
- Sclerostin and Cardiovascular Disease. | Tobias, JH. 2023. Curr Osteoporos Rep. 21: 519-526. PMID: 37490188
- Sclerostin: clinical insights in muscle-bone crosstalk. | Moretti, A. and Iolascon, G. 2023. J Int Med Res. 51: 3000605231193293. PMID: 37632438
- Sclerostin inhibition in rare bone diseases: Molecular understanding and therapeutic perspectives. | Xiaohui, T., et al. 2024. J Orthop Translat. 47: 39-49. PMID: 39007037
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sclerostin Antibody (C-11) | sc-518161 | 200 µg/ml | $322.00 | |||
Sclerostin Antibody (C-11): m-IgGκ BP-HRP Bundle | sc-550668 | 200 µg Ab; 40 µg BP | $361.00 | |||
Sclerostin Antibody (C-11) AC | sc-518161 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Sclerostin Antibody (C-11) HRP | sc-518161 HRP | 200 µg/ml | $322.00 | |||
Sclerostin Antibody (C-11) FITC | sc-518161 FITC | 200 µg/ml | $336.00 | |||
Sclerostin Antibody (C-11) PE | sc-518161 PE | 200 µg/ml | $349.00 | |||
Sclerostin Antibody (C-11) Alexa Fluor® 488 | sc-518161 AF488 | 200 µg/ml | $364.00 | |||
Sclerostin Antibody (C-11) Alexa Fluor® 546 | sc-518161 AF546 | 200 µg/ml | $364.00 | |||
Sclerostin Antibody (C-11) Alexa Fluor® 594 | sc-518161 AF594 | 200 µg/ml | $364.00 | |||
Sclerostin Antibody (C-11) Alexa Fluor® 647 | sc-518161 AF647 | 200 µg/ml | $364.00 | |||
Sclerostin Antibody (C-11) Alexa Fluor® 680 | sc-518161 AF680 | 200 µg/ml | $364.00 | |||
Sclerostin Antibody (C-11) Alexa Fluor® 790 | sc-518161 AF790 | 200 µg/ml | $364.00 |