SCG10 CRISPR/Cas9 KO Plasmid (m2): sc-422814-KO-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SCG10 CRISPR/Cas9 Knockout (KO) Plasmid (m2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SCG10 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SCG10 Antibody (2-RE19): sc-135620
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SCG10 CRISPR/Cas9 KO Plasmid (m2) | sc-422814-KO-2 | 20 µg | $397.00 |
Overview
Stmn2 encodes SCG10, a neuron-enriched member of the stathmin family that binds tubulin and promotes microtubule destabilization, supporting dynamic remodeling of the cytoskeleton during neurite outgrowth and axon guidance in mouse nervous system development. SCG10 activity influences growth cone behavior, intracellular trafficking, and activity-dependent plasticity by coupling signaling inputs to microtubule turnover. Dysregulation of STMN2/SCG10 has been linked to neuronal stress responses and altered axonal maintenance, making it relevant to studies of neurodegeneration and motor neuron vulnerability. In cellular models, perturbing SCG10 impacts neuronal differentiation programs, axonal regeneration phenotypes, and cytoskeleton-dependent synaptic processes.
SCG10 CRISPR/Cas9 KO Plasmid (m2) is a pool of plasmids designed for targeted disruption of the Stmn2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Stmn2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Stmn2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SCG10 protein expression.
This CRISPR knockout system enables efficient generation of Stmn2-deficient cell models for investigation of SCG10 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Stmn2 exon(s) critical for SCG10 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Stmn2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SCG10 CRISPR/Cas9 KO Plasmid (m) and SCG10 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Stmn2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SCG10 HDR Plasmid (m) and SCG10 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Stmn2 homology arms to support homology-directed repair at defined Stmn2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.