SCF Antibody (G-3): sc-13126

SCF Antibody (G-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects SCF protein of mouse, rat, and human origin by WB, IP, IF, IHCP, and ELISA. SCF Antibody (G-3) is available in both non-conjugated and various conjugated forms, including agarose, HRP, PE, FITC, and multiple Alexa Fluor® conjugates. SCF plays a crucial role as the ligand for the transmembrane tyrosine kinase receptor proto-oncogene c-Kit, which is vital for several cellular processes. SCF is a pleiotropic cytokine that exists in two alternatively spliced forms, consisting of 248 and 220 amino acids in human and mouse systems, respectively. The larger form of SCF is predominantly expressed in fibroblasts, brain, and thymus, while the smaller variant is found in tissues such as the spleen, testis, placenta, and cerebellum. SCF is essential for the development of germ cells, hematopoietic progenitor cells, and melanocyte precursors, as SCF stimulates the proliferation and maturation of both mature and immature mast cells. The interaction of SCF with c-Kit is critical for various biological processes, including hematopoiesis and pigmentation, making SCF monoclonal antibody (G-3) a valuable tool for research in developmental biology and cancer studies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SCF Antibody (G-3) References:

1. Differential expression and processing of two cell associated forms of the kit-ligand: KL-1 and KL-2.  |  Huang, EJ., et al. 1992. Mol Biol Cell. 3: 349-62. PMID: 1378327
2. Support of human hematopoiesis in long-term bone marrow cultures by murine stromal cells selectively expressing the membrane-bound and secreted forms of the human homolog of the steel gene product, stem cell factor.  |  Toksoz, D., et al. 1992. Proc Natl Acad Sci U S A. 89: 7350-4. PMID: 1380155
3. Cleavage of membrane-anchored growth factors involves distinct protease activities regulated through common mechanisms.  |  Pandiella, A., et al. 1992. J Biol Chem. 267: 24028-33. PMID: 1385433
4. Mast cell growth factor maps near the steel locus on mouse chromosome 10 and is deleted in a number of steel alleles.  |  Copeland, NG., et al. 1990. Cell. 63: 175-83. PMID: 1698554
5. Primary structure and functional expression of rat and human stem cell factor DNAs.  |  Martin, FH., et al. 1990. Cell. 63: 203-11. PMID: 2208279
6. Role of stem cell factor in the placental niche.  |  Khodadi, E., et al. 2016. Cell Tissue Res. 366: 523-531. PMID: 27234501
7. Erythropoietin, Stem Cell Factor, and Cancer Cell Migration.  |  Vazquez-Mellado, MJ., et al. 2017. Vitam Horm. 105: 273-296. PMID: 28629522
8. Bone marrow adipocytes promote the regeneration of stem cells and haematopoiesis by secreting SCF.  |  Zhou, BO., et al. 2017. Nat Cell Biol. 19: 891-903. PMID: 28714970
9. Stem cell factor: the bridge between bone marrow adipocytes and hematopoietic cells.  |  Li, Z. and MacDougald, OA. 2019. Haematologica. 104: 1689-1691. PMID: 31473604
10. Inhibition of Soluble Stem Cell Factor Promotes Intestinal Mucosal Repair.  |  Garcia-Hernandez, V., et al. 2023. Inflamm Bowel Dis. 29: 1133-1144. PMID: 36688460
11. Stem cell factor and cKIT modulate endothelial glycolysis in hypoxia.  |  Jeong, H., et al. 2024. Cardiovasc Res. 120: 745-755. PMID: 38507654
12. Monocytes do not make mast cells when cultured in the presence of SCF. Characterization of the circulating mast cell progenitor as a c-kit+, CD34+, Ly-, CD14-, CD17-, colony-forming cell.  |  Agis, H., et al. 1993. J Immunol. 151: 4221-7. PMID: 7691941

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SCF Antibody (G-3) has 53 citations in a variety of scientific publications.

Primary antibodies like Anti-SCF Antibody (G-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SCF Antibody (G-3) Specifications

Alternate Product Name: Stem Cell Factor

Product Clone Name: G-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 26-214

Epitope Target: SCF

Epitope Species: human

Product Reactivity/Detection: SCF

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 53 citations in various scientific publications

Available Conjugates/Forms for SCF Antibody (G-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin