Anti-SCCA1 Antibody (8H11) is a mouse monoclonal IgG1 (kappa light chain) SCCA1 antibody provided at 200 µg/ml
raised against recombinant human SCCA1
SCCA1 Antibody (8H11) is recommended for detection of SCCA1 of human origin by WB, IP, IF and IHC(P)
SCCA1 Antibody (8H11) is available conjugated to agarose for IP; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SCCA1 (8H11): sc-21767.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
SCCA1 Antibody (8H11) is a high quality monoclonal SCCA1 antibody (also designated SCCA1 antibody) suitable for the detection of the SCCA1 protein of human origin. SCCA1 Antibody (8H11) is available as both the non-conjugated anti-SCCA1 antibody form, as well as multiple conjugated forms of anti-SCCA1 antibody, including agarose, PE, FITC and multiple Alexa Fluor® conjugates. Metastasis of a primary tumor to a distant site is determined through signaling cascades that break down interactions between the cell and extracellular matrix proteins. Among the proteins mediating metastasis are serine prote-ases, such as neutrophil elastase. In 1985, Dr. Jim Travis and Dr. R.W. Carrell designated an emerging family of serine protease inhibitors as the serpin fam-ily, which share homology in both primary amino acid sequence and tertiary structure. Serpins contain a stretch of peptide that mimics a true substrate for a corresponding serine protease. Serine proteases bind to this substrate mimic in a 1:1 stoichiometric fashion and become catalytically inactive. Aberrant ex-pression of serpin family members can contribute to a number of conditions, including emphysema (a-1 antitrypsin deficiency), fatal bleeding (elastase to thrombin specificity) and thrombosis (antithrombin deficiency), and are indicators of cancer stage phenotypes (circulating levels of squamous cell carcinoma antigen, known as SCCA1, increase in advancing stages of some cervical, lung, esophageal and head and neck cancers). Human chromosome position 18q21.3 contains a cluster of serpins, including a tandem duplication of the SCCA gene, plasminogen activator inhibitor type 2, and maspin. SCCA is transcribed by two nearly identical genes (SCCA1 and SCCA2), and is mainly produced as SCCA1. The human SCCA1 gene encodes a 390 amino acid protein that was originally isolated from a metastatic cervical squamous cell carcinoma.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended secondary for use with this mouse monoclonal primary antibody?
Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Rated 5 out of
Good for WB and IHCSCCA1 Antibody (8H11) is a good choice for WB and IHC(P) as indicated by recent journal publications.
Date published: 2017-01-05
Rated 4 out of
Used this antibody in WB and got correct bandUsed this antibody in WB and got correct band.
Date published: 2015-08-26
Rated 5 out of
This antibody was used in a publication forThis antibody was used in a publication for WB on human esophageal squamous cell carcinoma - SCBT Publication Review
Date published: 2015-05-18
Rated 5 out of
Successful application of this antibody inSuccessful application of this antibody in WB using human saliva from patient with OSCC ulcerative lesions located on the tongue -SCBT Publication Review
Date published: 2015-05-06
Rated 5 out of
Publishable IHCP and WB data with human 293TPublishable IHCP and WB data with human 293T cells - SCBT Publication Review
Date published: 2015-02-14
Rated 5 out of
IHC publishable data with human normal peripheralIHC publishable data with human normal peripheral blood mononuclear cells and vulvar squamous cell - SCBT Publication Review
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn