Anti-SCCA1/2 Antibody (B-9) is a mouse monoclonal IgG1 (kappa light chain) SCCA1/2 antibody provided at 200 µg/ml
raised against amino acids 1-390 representing full length SCCA2 of human origin
Anti-SCCA1/2 Antibody (B-9) is recommended for detection of SCCA1 and SCCA2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Anti-SCCA1/2 Antibody (B-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SCCA1/2 (B-9): sc-28384.
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SCCA1/2 Antibody (B-9) is a high quality monoclonal SCCA1/2 antibody (also designated SCCA1/2 antibody) suitable for the detection of the SCCA1/2 protein of mouse, rat and human origin. SCCA1/2 Antibody (B-9) is available as both the non-conjugated anti-SCCA1/2 antibody form, as well as multiple conjugated forms of anti-SCCA1/2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Metastasis of a primary tumor to a distant site is determined through signaling cascades that break down interactions between the cell and extracellular matrix proteins. Among the proteins mediating metastasis are serine prote-ases, such as neutrophil elastase. In 1985, Dr. Jim Travis and Dr. R.W. Carrell designated an emerging family of serine protease inhibitors as the serpin fam-ily, which share homology in both primary amino acid sequence and tertiary structure. Serpins contain a stretch of peptide that mimics a true substrate for a corresponding serine protease. Serine proteases bind to this substrate mimic in a 1:1 stoichiometric fashion and become catalytically inactive. Aberrant ex-pression of serpin family members can contribute to a number of conditions, including emphysema (a-1 antitrypsin deficiency), fatal bleeding (elastase to thrombin specificity) and thrombosis (antithrombin deficiency), and are indicators of cancer stage phenotypes (circulating levels of squamous cell carcinoma antigen, known as SCCA1, increase in advancing stages of some cervical, lung, esophageal and head and neck cancers). Human chromosome position 18q21.3 contains a cluster of serpins, including a tandem duplication of the SCCA gene, plasminogen activator inhibitor type 2, and maspin. SCCA is transcribed by two nearly identical genes (SCCA1 and SCCA2), and is mainly produced as SCCA1. The human SCCA1 gene encodes a 390 amino acid protein that was originally isolated from a metastatic cervical squamous cell carcinoma.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Can SCCA1/2 (B-9): sc-28384 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?
Asked by: jenni
Thank you for your question. Yes, SCCA1/2 (B-9): sc-28384 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Rated 5 out of
Preferred replacement for FL-390SCCA1/2 mouse monoclonal antibody (B-9)clone is the preferred replacement for rabbit polyclonal (FL-390). It has the same epitope, but has confirmed specificity and reproducibility in Western blotting and immunohistochemistry applications.
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