SCCA1/2 Antibody (B-9) recommended for detection of SCCA1 and SCCA2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

SCCA1/2 Antibody (B-9): sc-28384

SCCA1/2 Antibody (B-9) is rated 5.0 out of 5 by 2.
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Datasheets

    • Anti-SCCA1/2 Antibody (B-9) is a mouse monoclonal IgG1 (kappa light chain) SCCA1/2 antibody provided at 200 µg/ml
    • raised against amino acids 1-390 representing full length SCCA2 of human origin
    • Anti-SCCA1/2 Antibody (B-9) is recommended for detection of SCCA1 and SCCA2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-SCCA1/2 Antibody (B-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SCCA1/2 (B-9): sc-28384.

Can SCCA1/2 (B-9): sc-28384 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, SCCA1/2 (B-9): sc-28384 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 14
Date published: 2017-02-07
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Rated 5 out of 5 by from Preferred replacement for FL-390 SCCA1/2 mouse monoclonal antibody (B-9)clone is the preferred replacement for rabbit polyclonal (FL-390). It has the same epitope, but has confirmed specificity and reproducibility in Western blotting and immunohistochemistry applications.
Date published: 2017-01-23
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