Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SCCA1/2 CRISPR/Cas9 KO Plasmid (h): sc-418018
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SCCA1/2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SCCA1/2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SCCA1/2 Antibody (B-9): sc-28384
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SCCA1/2 CRISPR/Cas9 KO Plasmid (h) | sc-418018 | 20 µg | $397.00 |
Overview
SERPINB4 encodes squamous cell carcinoma antigen 1/2 (SCCA1/2), a clade B serine protease inhibitor that modulates intracellular proteolysis by inhibiting select cathepsins and related proteases. By restraining protease-driven protein turnover and inflammatory protease activity, SCCA1/2 influences epithelial differentiation, barrier-associated stress responses, and cytokine-linked signaling programs. Altered SERPINB4 expression has been reported in squamous epithelia and inflammatory microenvironments, where it is used as a biomarker in studies of tumor biology and chronic inflammation. Functional interrogation of SERPINB4 supports mechanistic work on protease–antiprotease balance, cell survival under stress, and crosstalk between epithelial and immune pathways.
SCCA1/2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SERPINB4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SERPINB4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SERPINB4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SCCA1/2 protein expression.
This CRISPR knockout system enables efficient generation of SERPINB4-deficient cell models for investigation of SCCA1/2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SERPINB4 exon(s) critical for SCCA1/2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SERPINB4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SCCA1/2 CRISPR/Cas9 KO Plasmid (h) and SCCA1/2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SERPINB4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SCCA1/2 HDR Plasmid (h) and SCCA1/2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SERPINB4 homology arms to support homology-directed repair at defined SERPINB4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.