SCCA1/2 Antibody (B-9): sc-28384

SCCA1/2 Antibody (B-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects SCCA1/2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-SCCA1/2 antibody (B-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SCCA1 and SCCA2 proteins, which are part of the serpin family of serine protease inhibitors, play a crucial role in regulating proteolytic activity in the extracellular matrix, thereby influencing processes such as tumor progression and metastasis. SCCA1 and SCCA2 proteins are primarily located in the cytoplasm and are secreted into the extracellular space, where SCCA1 and SCCA2 interact with serine proteases to inhibit their activity. Such inhibition is vital for maintaining tissue homeostasis and preventing excessive degradation of the extracellular matrix, which can lead to tumor invasion and metastasis. SCCA1, which encodes a 390 amino acid protein, was initially identified in metastatic cervical squamous cell carcinoma, and SCCA1 expression levels have been associated with various cancer stages, making SCCA1 a potential biomarker for cancer diagnosis and prognosis. SCCA1/2 proteins are encoded by two nearly identical genes located on human chromosome 18q21.3, and SCCA1/2 aberrant expression can indicate the presence and progression of several malignancies, including cervical, lung, esophageal, and head and neck cancers.

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SCCA1/2 Antibody (B-9) References:

1. Development of specific monoclonal antibodies and a sensitive discriminatory immunoassay for the circulating tumor markers SCCA1 and SCCA2.  |  Cataltepe, S., et al. 2000. Clin Chim Acta. 295: 107-27. PMID: 10767398
2. Circulating serpin tumor markers SCCA1 and SCCA2 are not actively secreted but reside in the cytosol of squamous carcinoma cells.  |  Uemura, Y., et al. 2000. Int J Cancer. 89: 368-77. PMID: 10956412
3. Relative levels of SCCA2 and SCCA1 mRNA in primary tumors predicts recurrent disease in squamous cell cancer of the head and neck.  |  Stenman, J., et al. 2001. Int J Cancer. 95: 39-43. PMID: 11241309
4. Effect of SCCA1 and SCCA2 on the suppression of TNF-alpha-induced cell death by impeding the release of mitochondrial cytochrome c in an oral squamous cell carcinoma cell line.  |  Hashimoto, K., et al. 2005. Tumour Biol. 26: 165-72. PMID: 16006770
5. Differential effects of periopathogens on host protease inhibitors SLPI, elafin, SCCA1, and SCCA2.  |  Yin, L., et al. 2010. J Oral Microbiol. 2: PMID: 21523231
6. Squamous Cell Carcinoma Antigen 2 (SCCA2, SERPINB4): An Emerging Biomarker for Skin Inflammatory Diseases.  |  Izuhara, K., et al. 2018. Int J Mol Sci. 19: PMID: 29642409
7. Salivary Scca1, Scca2 and Trop2 in Oral Cancer Patients-A Cross-Sectional Pilot Study.  |  Karmelić, I., et al. 2022. Dent J (Basel). 10: PMID: 35448064
8. SCCA1/SERPINB3 suppresses antitumor immunity and blunts therapy-induced T cell responses via STAT-dependent chemokine production.  |  Chen, L., et al. 2023. J Clin Invest. 133: PMID: 37279067
9. SCCA1 and SCCA2 are proteinase inhibitors that map to the serpin cluster at 18q21.3.  |  Silverman, GA., et al. 1998. Tumour Biol. 19: 480-7. PMID: 9817977
10. An 18q- syndrome breakpoint resides between the duplicated serpins SCCA1 and SCCA2 and arises via a cryptic rearrangement with satellite III DNA.  |  Katz, SG., et al. 1999. Hum Mol Genet. 8: 87-92. PMID: 9887335

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SCCA1/2 Antibody (B-9) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-SCCA1/2 Antibody (B-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SCCA1/2 Antibody (B-9) Specifications

Alternate Product Name: SCCA1/2

Product Clone Name: B-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-390

Epitope Target: SCCA2

Epitope Species: human

Product Reactivity/Detection: SCCA1 and SCCA2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for SCCA1/2 Antibody (B-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred SCCA1 antibody is SCCA1 Antibody (8H11): sc-21767.