SCAP Antibody (9D5): sc-13553

SCAP Antibody (9D5) is a mouse monoclonal IgG2b kappa light chain antibody that detects the SCAP protein of hamster origin by western blotting (WB) and immunoprecipitation (IP). SCAP (9D5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SCAP plays a crucial role in cholesterol homeostasis by acting as a sterol sensor that regulates the cleavage and activation of sterol regulatory element-binding proteins (SREBPs). Located in the endoplasmic reticulum, SCAP binds to the C-terminal regulatory domains of SREBPs, controlling their proteolytic activation in response to cellular sterol levels. When sterol levels are low, SCAP facilitates the cleavage of SREBPs at Site-1, allowing the N-terminal domain to translocate to the nucleus and induce the expression of genes involved in cholesterol metabolism. This regulatory mechanism is vital for maintaining lipid balance within cells, as dysregulation can lead to metabolic disorders. Anti-SCAP antibody (9D5) is an essential tool for researchers studying lipid metabolism and the intricate signaling pathways that govern cholesterol homeostasis.

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SCAP Antibody (9D5) References:

1. Autocatalytic processing of site-1 protease removes propeptide and permits cleavage of sterol regulatory element-binding proteins.  |  Espenshade, PJ., et al. 1999. J Biol Chem. 274: 22795-804. PMID: 10428864
2. Secreted site-1 protease cleaves peptides corresponding to luminal loop of sterol regulatory element-binding proteins.  |  Cheng, D., et al. 1999. J Biol Chem. 274: 22805-12. PMID: 10428865
3. A proteolytic pathway that controls the cholesterol content of membranes, cells, and blood.  |  Brown, MS. and Goldstein, JL. 1999. Proc Natl Acad Sci U S A. 96: 11041-8. PMID: 10500120
4. Sterols regulate cycling of SREBP cleavage-activating protein (SCAP) between endoplasmic reticulum and Golgi.  |  Nohturfft, A., et al. 1999. Proc Natl Acad Sci U S A. 96: 11235-40. PMID: 10500160
5. Glucose-Mediated N-glycosylation of SCAP Is Essential for SREBP-1 Activation and Tumor Growth.  |  Cheng, C., et al. 2015. Cancer Cell. 28: 569-581. PMID: 26555173
6. Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages.  |  Guo, C., et al. 2018. Immunity. 49: 842-856.e7. PMID: 30366764
7. Macrophage SCAP Contributes to Metaflammation and Lean NAFLD by Activating STING-NF-κB Signaling Pathway.  |  Huang, X., et al. 2022. Cell Mol Gastroenterol Hepatol. 14: 1-26. PMID: 35367665
8. Inhibiting SCAP/SREBP exacerbates liver injury and carcinogenesis in murine nonalcoholic steatohepatitis.  |  Kawamura, S., et al. 2022. J Clin Invest. 132: PMID: 35380992
9. Regulated cleavage of sterol regulatory element binding proteins requires sequences on both sides of the endoplasmic reticulum membrane.  |  Hua, X., et al. 1996. J Biol Chem. 271: 10379-84. PMID: 8626610
10. Cleavage of sterol regulatory element-binding proteins (SREBPs) at site-1 requires interaction with SREBP cleavage-activating protein. Evidence from in vivo competition studies.  |  Sakai, J., et al. 1998. J Biol Chem. 273: 5785-93. PMID: 9488713
11. Complementation cloning of S2P, a gene encoding a putative metalloprotease required for intramembrane cleavage of SREBPs.  |  Rawson, RB., et al. 1997. Mol Cell. 1: 47-57. PMID: 9659902
12. Molecular identification of the sterol-regulated luminal protease that cleaves SREBPs and controls lipid composition of animal cells.  |  Sakai, J., et al. 1998. Mol Cell. 2: 505-14. PMID: 9809072

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SCAP Antibody (9D5) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-SCAP Antibody (9D5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SCAP Antibody (9D5) Specifications

Alternate Product Name: SREBP Cleavage Activating Protein

Product Clone Name: 9D5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 540-707

Epitope Target: SCAP

Epitope Species: hamster

Product Reactivity/Detection: SCAP

Species Reactivity/Detection: hamster

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for SCAP Antibody (9D5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin