SATB1 Antibody (C-6): sc-376096

SATB1 Antibody (C-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects SATB1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SATB1 (C-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SATB1 protein, also known as Special AT-Rich Sequence Binding Protein 1, plays a crucial role in regulating gene expression, particularly during cell development and differentiation. SATB1 is primarily expressed in thymocytes and contains a unique homeobox DNA-binding domain essential for DNA interaction. SATB1′s structure includes an additional domain exhibiting high affinity for DNA, allowing specific binding to nuclear matrix attachment regions (MARs) of DNA. These MARs are critical for organizing chromosomal loops and regulating transcription and replication within the nucleus. SATB1′s interaction with MARs is vital for maintaining genome structural integrity and ensuring proper gene expression, making SATB1 an important factor in cellular function and development. SATB1′s ability to synergistically interact with other transcriptional repressors, such as CCAAT displacement protein (CDP), further underscores SATB1′s significance in mediating complex gene regulatory networks.

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SATB1 Antibody (C-6) References:

1. Homeoproteins CDP and SATB1 interact: potential for tissue-specific regulation.  |  Liu, J., et al. 1999. Mol Cell Biol. 19: 4918-26. PMID: 10373541
2. Modulated binding of SATB1, a matrix attachment region protein, to the AT-rich sequence flanking the major breakpoint region of BCL2.  |  Ramakrishnan, M., et al. 2000. Mol Cell Biol. 20: 868-77. PMID: 10629043
3. The MAR-binding protein SATB1 orchestrates temporal and spatial expression of multiple genes during T-cell development.  |  Alvarez, JD., et al. 2000. Genes Dev. 14: 521-35. PMID: 10716941
4. Special AT-rich Sequence-binding Protein 1 (SATB1) Functions as an Accessory Factor in Base Excision Repair.  |  Kaur, S., et al. 2016. J Biol Chem. 291: 22769-22780. PMID: 27590341
5. The Special AT-rich Sequence Binding Protein 1 (SATB1) and its role in solid tumors.  |  Frömberg, A., et al. 2018. Cancer Lett. 417: 96-111. PMID: 29306014
6. Special AT-rich Sequence Binding-Protein 1 (SATB1) Correlates with Immune Infiltration in Breast, Head and Neck, and Prostate Cancer.  |  Ge, H., et al. 2020. Med Sci Monit. 26: e923208. PMID: 32562536
7. A microRNA-21-mediated SATB1/S100A9/NF-κB axis promotes chronic obstructive pulmonary disease pathogenesis.  |  Kim, RY., et al. 2021. Sci Transl Med. 13: eaav7223. PMID: 34818056
8. TGF-β-mediated silencing of genomic organizer SATB1 promotes Tfh cell differentiation and formation of intra-tumoral tertiary lymphoid structures.  |  Chaurio, RA., et al. 2022. Immunity. 55: 115-128.e9. PMID: 35021053
9. An atypical homeodomain in SATB1 promotes specific recognition of the key structural element in a matrix attachment region.  |  Dickinson, LA., et al. 1997. J Biol Chem. 272: 11463-70. PMID: 9111059
10. Interaction of the nuclear matrix-associated region (MAR)-binding proteins, SATB1 and CDP/Cux, with a MAR element (L2a) in an upstream regulatory region of the mouse CD8a gene.  |  Banan, M., et al. 1997. J Biol Chem. 272: 18440-52. PMID: 9218488
11. The genomic sequences bound to special AT-rich sequence-binding protein 1 (SATB1) in vivo in Jurkat T cells are tightly associated with the nuclear matrix at the bases of the chromatin loops.  |  de Belle, I., et al. 1998. J Cell Biol. 141: 335-48. PMID: 9548713
12. A nuclear matrix attachment region upstream of the T cell receptor beta gene enhancer binds Cux/CDP and SATB1 and modulates enhancer-dependent reporter gene expression but not endogenous gene expression.  |  Chattopadhyay, S., et al. 1998. J Biol Chem. 273: 29838-46. PMID: 9792700

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SATB1 Antibody (C-6) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-SATB1 Antibody (C-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SATB1 Antibody (C-6) Specifications

Alternate Product Name: SATB Homeobox 1

Product Clone Name: C-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 241-310

Epitope Target: SATB1

Epitope Species: human

Product Reactivity/Detection: SATB1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for SATB1 Antibody (C-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent