SAS-6 Antibody (G-1) recommended for detection of SAS-6 of human origin by WB, IP, IF and ELISA

SAS-6 Antibody (G-1): sc-376836

SAS-6 Antibody (G-1) is rated 5.0 out of 5 by 2.
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Datasheets

    • SAS-6 Antibody (G-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of SAS-6 of human origin
    • recommended for detection of SAS-6 of human origin by WB, IP, IF and ELISA
    • available conjugated to agarose for IP
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See SAS-6 (91.390.21): sc-81431 for SAS-6 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What is the recommended fixation method for immunofluorescence staining with SAS-6 (G-1): sc-376836 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Produced beautiful immunofluorescence cytoplasmic Produced beautiful immunofluorescence cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-03-15
Rated 5 out of 5 by from Great Western Blot data of SAS Great Western Blot data of SAS-6 expression in MOLT-4 whole cell lysate. -SCBT QC
Date published: 2013-05-11
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