SAS-6 Antibody (91.390.21): sc-81431

SAS-6 Antibody (91.390.21) is a mouse monoclonal IgG2b kappa light chain antibody that detects SAS-6 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-SAS-6 antibody (91.390.21) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SAS-6 protein, also known as spindle assembly abnormal protein 6 homolog, is a crucial 657 amino acid component of the centrosome, characterized by the presence of a PISA (present in SAS-6) domain. SAS-6 (91.390.21) monoclonal antibody recognizes a protein that plays a vital role in centriole formation, essential for proper cell division and organization of the mitotic spindle. SAS-6 protein is specifically localized asymmetrically next to the centriole during procentriole formation onset, and SAS-6 levels fluctuate throughout the cell cycle, being degraded during mitosis and accumulating again at the end of the G1 phase. SAS-6 degradation is mediated by the anaphase-promoting complex, which targets SAS-6 for destruction via the 26S proteasome, with a KEN box in its C-terminus being critical for this process. Increased SAS-6 levels can lead to multiple procentriole formation adjacent to a single centriole, highlighting SAS-6 importance in centriole duplication and cellular integrity maintenance.

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SAS-6 Antibody (91.390.21) References:

1. SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells.  |  Leidel, S., et al. 2005. Nat Cell Biol. 7: 115-25. PMID: 15665853
2. Centrosome duplication and nematodes: recent insights from an old relationship.  |  Leidel, S. and Gönczy, P. 2005. Dev Cell. 9: 317-25. PMID: 16139223
3. Sequential protein recruitment in C. elegans centriole formation.  |  Delattre, M., et al. 2006. Curr Biol. 16: 1844-9. PMID: 16979563
4. Centriole assembly in Caenorhabditis elegans.  |  Pelletier, L., et al. 2006. Nature. 444: 619-23. PMID: 17136092
5. Overexpressing centriole-replication proteins in vivo induces centriole overduplication and de novo formation.  |  Peel, N., et al. 2007. Curr Biol. 17: 834-43. PMID: 17475495
6. Molecular characterization of centriole assembly in ciliated epithelial cells.  |  Vladar, EK. and Stearns, T. 2007. J Cell Biol. 178: 31-42. PMID: 17606865
7. DSAS-6 organizes a tube-like centriole precursor, and its absence suggests modularity in centriole assembly.  |  Rodrigues-Martins, A., et al. 2007. Curr Biol. 17: 1465-72. PMID: 17689959
8. SAS-6 assembly templated by the lumen of cartwheel-less centrioles precedes centriole duplication.  |  Fong, CS., et al. 2014. Dev Cell. 30: 238-45. PMID: 25017693
9. The PLK4-STIL-SAS-6 module at the core of centriole duplication.  |  Arquint, C. and Nigg, EA. 2016. Biochem Soc Trans. 44: 1253-1263. PMID: 27911707
10. Pericentrin-mediated SAS-6 recruitment promotes centriole assembly.  |  Ito, D., et al. 2019. Elife. 8: PMID: 31182187
11. Identification of compounds that bind the centriolar protein SAS-6 and inhibit its oligomerization.  |  Busch, JMC., et al. 2020. J Biol Chem. 295: 17922-17934. PMID: 32873708
12. Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly.  |  Hatzopoulos, GN., et al. 2021. Nat Commun. 12: 3805. PMID: 34155202

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SAS-6 Antibody (91.390.21) has 85 citations in a variety of scientific publications.

Primary antibodies like Anti-SAS-6 Antibody (91.390.21) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SAS-6 Antibody (91.390.21) Specifications

Alternate Product Name: SAS-6

Product Clone Name: 91.390.21

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 404-657

Epitope Target: SAS-6

Epitope Species: human

Product Reactivity/Detection: SAS-6

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 85 citations in various scientific publications

Available Conjugates/Forms for SAS-6 Antibody (91.390.21): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin