SART-1 Antibody (A-5): sc-376460
- SART-1 Antibody (A-5) is a mouse monoclonal IgG2a κ SART-1 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 107-406 mapping near the N-terminus of SART-1 of human origin
- SART-1 Antibody (A-5) is recommended for detection of SART-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-SART-1 Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SART-1 Antibody (A-5) for WB and IHC(P) applications. These reagents are now offered in bundles with SART-1 Antibody (A-5) (see ordering information below).
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SART-1 Antibody (A-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects SART-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SART-1 (A-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SART-1, also known as squamous cell carcinoma antigen recognized by T cells, Ara1, HOMS1, or Snu66, plays a crucial role in mRNA splicing and cell proliferation, making SART-1 significant in cancer biology and therapeutic research. SART-1 protein is primarily localized in the nucleus of proliferating cells, where SART-1 is involved in gene expression regulation and spliceosome assembly, a complex essential for pre-mRNA splicing. Notably, SART-1 undergoes post-transcriptional modifications that yield two distinct protein isoforms: SART-1(800), which is the native form, and SART-1(259), which is expressed in epithelial cancer cytosol. While both isoforms contribute to cell proliferation, SART-1(259) contains a tumor-rejection antigen that can stimulate cytotoxic T lymphocytes in cancer patients, highlighting SART-1′s potential in immunotherapy. Furthermore, SART-1 gene variations may link to atopy, a condition characterized by allergic hypersensitivity, including eczema, asthma, and allergic conjunctivitis, underscoring SART-1′s relevance in both cancer and allergy research.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SART-1 Antibody (A-5) References:
- Expression of the SART-1 antigens in uterine cancers. | Matsumoto, H., et al. 1998. Jpn J Cancer Res. 89: 1292-5. PMID: 10081490
- Intron loss in the SART1 genes of Fugu rubripes and Tetraodon nigroviridis. | Bolland, DJ. and Hewitt, JE. 2001. Gene. 271: 43-9. PMID: 11410364
- Identification of the autoantigen SART-1 as a candidate gene for the development of atopy. | Wheatley, AP., et al. 2002. Hum Mol Genet. 11: 2143-6. PMID: 12189166
- Generation of a human leukocyte antigen-A24-restricted antitumor cell with the use of SART-1 peptide and dendritic cells in patients with malignant brain tumors. | Yoshida, S. and Tanaka, R. 2004. J Lab Clin Med. 144: 201-7. PMID: 15514588
- Cell cycle arrest and apoptosis induced by SART-1 gene transduction. | Hosokawa, M., et al. 2005. Anticancer Res. 25: 1983-90. PMID: 16158934
- Essential domains for ribonucleoprotein complex formation required for retrotransposition of telomere-specific non-long terminal repeat retrotransposon SART1. | Matsumoto, T., et al. 2006. Mol Cell Biol. 26: 5168-79. PMID: 16782900
- Expression of the SART-1 tumor rejection antigen in breast cancer. | Kawamoto, M., et al. 1999. Int J Cancer. 80: 64-7. PMID: 9935232
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SART-1 Antibody (A-5) | sc-376460 | 200 µg/ml | $322.00 | |||
SART-1 Antibody (A-5): m-IgG Fc BP-HRP Bundle | sc-529870 | 200 µg Ab; 10 µg BP | $361.00 | |||
SART-1 Antibody (A-5): m-IgGκ BP-HRP Bundle | sc-522958 | 200 µg Ab, 40 µg BP | $361.00 | |||
SART-1 Antibody (A-5): m-IgG2a BP-HRP Bundle | sc-547394 | 200 µg Ab; 10 µg BP | $361.00 | |||
SART-1 Antibody (A-5) AC | sc-376460 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SART-1 Antibody (A-5) HRP | sc-376460 HRP | 200 µg/ml | $322.00 | |||
SART-1 Antibody (A-5) FITC | sc-376460 FITC | 200 µg/ml | $336.00 | |||
SART-1 Antibody (A-5) PE | sc-376460 PE | 200 µg/ml | $349.00 | |||
SART-1 Antibody (A-5) Alexa Fluor® 488 | sc-376460 AF488 | 200 µg/ml | $364.00 | |||
SART-1 Antibody (A-5) Alexa Fluor® 546 | sc-376460 AF546 | 200 µg/ml | $364.00 | |||
SART-1 Antibody (A-5) Alexa Fluor® 594 | sc-376460 AF594 | 200 µg/ml | $364.00 | |||
SART-1 Antibody (A-5) Alexa Fluor® 647 | sc-376460 AF647 | 200 µg/ml | $364.00 | |||
SART-1 Antibody (A-5) Alexa Fluor® 680 | sc-376460 AF680 | 200 µg/ml | $364.00 | |||
SART-1 Antibody (A-5) Alexa Fluor® 790 | sc-376460 AF790 | 200 µg/ml | $364.00 |