SAP-1a Antibody (H-3): sc-166823
- SAP-1a Antibody (H-3) is a mouse monoclonal IgG2a κ SAP-1a antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 154-320 of SAP-1a of human origin
- SAP-1a Antibody (H-3) is recommended for detection of SAP-1a and SAP-1b of human origin by WB, IP, IF and ELISA
- Anti-SAP-1a Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-166823 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SAP-1a Antibody (H-3) for WB applications. These reagents are now offered in bundles with SAP-1a Antibody (H-3) (see ordering information below).
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SAP-1a Antibody (H-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects SAP-1a protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SAP-1a (H-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SAP-1a protein, also known as serum response factor accessory protein-1, plays a crucial role in regulating gene expression in response to growth factors by forming a ternary complex with serum response factor (SRF) and other proteins. This interaction is vital for transcriptional activation of genes involved in cell growth and differentiation, particularly in muscle cells, where SRF-binding sites are essential for muscle-specific gene expression. SAP-1a′s ability to interact with SRF and other Ets domain proteins, such as Elk-1, underscores its importance in signal transduction pathways that mediate cellular responses to external stimuli. Additionally, conserved phosphorylation sites within SAP-1a allow for post-translational modifications that can further regulate activity and interactions, making SAP-1a a significant player in cellular signaling networks.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SAP-1a Antibody (H-3) References:
- Ternary complex factors Elk-1 and Sap-1a mediate growth hormone-induced transcription of egr-1 (early growth response factor-1) in 3T3-F442A preadipocytes. | Clarkson, RW., et al. 1999. Mol Endocrinol. 13: 619-31. PMID: 10194767
- Characterization of SAP-1, a protein recruited by serum response factor to the c-fos serum response element. | Dalton, S. and Treisman, R. 1992. Cell. 68: 597-612. PMID: 1339307
- Spatial flexibility in ternary complexes between SRF and its accessory proteins. | Treisman, R., et al. 1992. EMBO J. 11: 4631-40. PMID: 1425594
- elk-1 domains responsible for autonomous DNA binding, SRE:SRF interaction and negative regulation of DNA binding. | Rao, VN. and Reddy, ES. 1992. Oncogene. 7: 2335-40. PMID: 1437156
- Epidermal growth factor and other mitogens induce binding of a protein complex to the c-fos serum response element in human astrocytoma and other cells. | Malik, RK., et al. 1991. J Biol Chem. 266: 8576-82. PMID: 1902475
- The SRE: a growth factor responsive transcriptional regulator. | Treisman, R. 1990. Semin Cancer Biol. 1: 47-58. PMID: 2133110
- The sarcomeric actin CArG-binding factor is indistinguishable from the c-fos serum response factor. | Boxer, LM., et al. 1989. Mol Cell Biol. 9: 515-22. PMID: 2710114
- Isolation and properties of cDNA clones encoding SRF, a transcription factor that binds to the c-fos serum response element. | Norman, C., et al. 1988. Cell. 55: 989-1003. PMID: 3203386
- Functional analysis of a growth factor-responsive transcription factor complex. | Hill, CS., et al. 1993. Cell. 73: 395-406. PMID: 8477450
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SAP-1a Antibody (H-3) | sc-166823 | 200 µg/ml | $322.00 | |||
SAP-1a Antibody (H-3): m-IgG Fc BP-HRP Bundle | sc-529060 | 200 µg Ab; 10 µg BP | $361.00 | |||
SAP-1a Antibody (H-3): m-IgGκ BP-HRP Bundle | sc-521759 | 200 µg Ab, 40 µg BP | $361.00 | |||
SAP-1a Antibody (H-3): m-IgG2a BP-HRP Bundle | sc-547251 | 200 µg Ab; 10 µg BP | $361.00 | |||
SAP-1a Antibody (H-3) X | sc-166823 X | 200 µg/0.1 ml | $322.00 | |||
SAP-1a Antibody (H-3) AC | sc-166823 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SAP-1a Antibody (H-3) HRP | sc-166823 HRP | 200 µg/ml | $322.00 | |||
SAP-1a Antibody (H-3) FITC | sc-166823 FITC | 200 µg/ml | $336.00 | |||
SAP-1a Antibody (H-3) PE | sc-166823 PE | 200 µg/ml | $349.00 | |||
SAP-1a Antibody (H-3) Alexa Fluor® 488 | sc-166823 AF488 | 200 µg/ml | $364.00 | |||
SAP-1a Antibody (H-3) Alexa Fluor® 546 | sc-166823 AF546 | 200 µg/ml | $364.00 | |||
SAP-1a Antibody (H-3) Alexa Fluor® 594 | sc-166823 AF594 | 200 µg/ml | $364.00 | |||
SAP-1a Antibody (H-3) Alexa Fluor® 647 | sc-166823 AF647 | 200 µg/ml | $364.00 | |||
SAP-1a Antibody (H-3) Alexa Fluor® 680 | sc-166823 AF680 | 200 µg/ml | $364.00 | |||
SAP-1a Antibody (H-3) Alexa Fluor® 790 | sc-166823 AF790 | 200 µg/ml | $364.00 |