Sam 68 Antibody (H-4): sc-514468

Sam 68 (H-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Sam 68 of mouse, rat and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). Anti-Sam 68 antibody (H-4) is available as non-conjugated form, as well as multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. Sam 68 is phosphorylated on tyrosine and functions as a substrate for Src family tyrosine kinases during mitosis. Sam 68 associates with several SH2 and SH3 domain-containing signaling proteins, such as GRB2 and PLC γ1. Originally cloned as Ras GAP-associated p62, further investigations have shown that Sam 68 and Ras GAP-associated p62 are not antigenically related, nor are they encoded by the same gene. Like Sam 68, Sam 68-like mammalian proteins, SLM-1 and SLM-2, demonstrate RNA binding activity. SLM-1 is tyrosine phosphorylated and functions as an adapter protein for signaling molecules, including GRB2, PLC γ1, Fyn and Ras GAP. SLM-2 is not tyrosine phosphorylated, nor does SLM-2 appear to associate with GRB2, PLC γ1, Fyn or Ras GAP, indicating a different role in protein interactions.

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Sam 68 Antibody (H-4) References:

1. Ablation of the Sam68 RNA binding protein protects mice from age-related bone loss.  |  Richard, S., et al. 2005. PLoS Genet. 1: e74. PMID: 16362077
2. High Sam68 expression predicts poor prognosis in non-small cell lung cancer.  |  Zhang, Z., et al. 2014. Clin Transl Oncol. 16: 886-91. PMID: 24522888
3. Decreased expression of SAM68 in human testes with spermatogenic defects.  |  Li, LJ., et al. 2014. Fertil Steril. 102: 61-67.e3. PMID: 24794312
4. Sam68 Regulates S6K1 Alternative Splicing during Adipogenesis.  |  Song, J. and Richard, S. 2015. Mol Cell Biol. 35: 1926-39. PMID: 25776557
5. Inhibition of Sam68 triggers adipose tissue browning.  |  Zhou, J., et al. 2015. J Endocrinol. 225: 181-9. PMID: 25934704
6. Sam68 promotes cellular proliferation and predicts poor prognosis in esophageal squamous cell carcinoma.  |  Wang, Y., et al. 2015. Tumour Biol. 36: 8735-45. PMID: 26050229
7. Sam68 impedes the recovery of arterial injury by augmenting inflammatory response.  |  Han, S., et al. 2019. J Mol Cell Cardiol. 137: 82-92. PMID: 31639388
8. Effective targeting of breast cancer stem cells by combined inhibition of Sam68 and Rad51.  |  Turdo, A., et al. 2022. Oncogene. 41: 2196-2209. PMID: 35217791
9. Decreased Expression of Sam68 Is Associated with Insulin Resistance in Granulosa Cells from PCOS Patients.  |  Vilariño-García, T., et al. 2022. Cells. 11: PMID: 36139396
10. Validating Sam68 expression and protein level in breast cancer.  |  Khudhair, MK., et al. 2023. J Med Life. 16: 1136-1139. PMID: 37900073

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Sam 68 Antibody (H-4) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Sam 68 Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Sam 68 Antibody (H-4) Specifications

Alternate Product Name: KHDRBS1

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 416-443

Epitope Target: Sam 68

Epitope Species: human

Product Reactivity/Detection: Sam 68

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Sam 68 Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Sam 68 antibody is Sam 68 Antibody (7-1): sc-1238.