Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SA-2 CRISPR/Cas9 KO Plasmid (h): sc-401222
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SA-2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SA-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SA-2 Antibody (G-12): sc-398229
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SA-2 CRISPR/Cas9 KO Plasmid (h) | sc-401222 | 20 µg | $397.00 |
Overview
STAG2 encodes the human cohesin subunit SA-2, a core component of the cohesin complex that mediates sister chromatid cohesion and higher-order genome organization. SA-2 supports faithful chromosome segregation during mitosis and meiosis, contributes to DNA double-strand break repair, and helps regulate long-range enhancer–promoter interactions that shape transcriptional programs. Through these roles, STAG2 intersects with cell-cycle control, replication stress responses, and chromatin architecture pathways. Disruption of STAG2 is frequently observed in cancer genomics and is associated with chromosomal instability and altered gene regulation, making it a widely studied locus in genome maintenance research.
SA-2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the STAG2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the STAG2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the STAG2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SA-2 protein expression.
This CRISPR knockout system enables efficient generation of STAG2-deficient cell models for investigation of SA-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting STAG2 exon(s) critical for SA-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple STAG2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SA-2 CRISPR/Cas9 KO Plasmid (h) and SA-2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the STAG2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SA-2 HDR Plasmid (h) and SA-2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by STAG2 homology arms to support homology-directed repair at defined STAG2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.