SA-2 Antibody (G-12): sc-398229

SA-2 Antibody (G-12) is a mouse monoclonal IgG3 kappa light chain antibody that detects SA-2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-SA-2 antibody (G-12) is available as the non-conjugated form. SA-2, also known as STAG2, is a crucial component of the cohesion complex, which is essential for maintaining sister chromatid cohesion following DNA replication. This multi-protein structure plays a vital role in ensuring accurate chromosome segregation during cell division and contributes to mitotic spindle assembly. SA-2, comprising 1,231 amino acids, interacts directly with Rad21, another key protein in the cohesion complex, and localizes in the nucleus where SA-2 associates with chromatin. Upon phosphorylation by Polo-like kinase (Plk), SA-2 dissociates from chromatin, facilitating proper chromosome separation during anaphase. SA-2 has been shown to enhance tumor necrosis factor alpha (TNFα) activity, suggesting a potential role as a transcriptional regulator, which may have implications in cancer biology and therapeutic strategies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SA-2 Antibody (G-12) References:

1. STAG2 expression in oral cancer and potentially malignant lesions.  |  Bernardes, VF., et al. 2014. Tumour Biol. 35: 3641-5. PMID: 24318971
2. Loss of cohesin complex components STAG2 or STAG3 confers resistance to BRAF inhibition in melanoma.  |  Shen, CH., et al. 2016. Nat Med. 22: 1056-61. PMID: 27500726
3. Synthetic lethal interaction between the tumour suppressor STAG2 and its paralog STAG1.  |  Benedetti, L., et al. 2017. Oncotarget. 8: 37619-37632. PMID: 28430577
4. Loss of Stromal Antigen 2 (STAG2) Expression in Upper Urinary Tract Carcinoma: Differential Prognostic Effect According to the Ki-67 Proliferating Index.  |  Miyakawa, J., et al. 2017. Ann Surg Oncol. 24: 4059-4066. PMID: 28967037
5. STAG2 Is a Biomarker for Prediction of Recurrence and Progression in Papillary Non-Muscle-Invasive Bladder Cancer.  |  Lelo, A., et al. 2018. Clin Cancer Res. 24: 4145-4153. PMID: 29954776
6. Loss of Stag2 cooperates with EWS-FLI1 to transform murine Mesenchymal stem cells.  |  El Beaino, M., et al. 2020. BMC Cancer. 20: 3. PMID: 31898537
7. STAG1 vulnerabilities for exploiting cohesin synthetic lethality in STAG2-deficient cancers.  |  van der Lelij, P., et al. 2020. Life Sci Alliance. 3: PMID: 32467316
8. Autoimmune manifestations in STAG2-mutated myeloid neoplasms.  |  Katamesh, B., et al. 2022. Ann Hematol. 101: 2785-2787. PMID: 36184685
9. Neuroprotection of Rodent and Human Retinal Ganglion Cells In Vitro/Ex Vivo by the Hybrid Small Molecule SA-2.  |  Pham, JH., et al. 2022. Cells. 11: PMID: 36497005
10. Modulation of Mitochondrial Metabolic Parameters and Antioxidant Enzymes in Healthy and Glaucomatous Trabecular Meshwork Cells with Hybrid Small Molecule SA-2.  |  Amankwa, CE., et al. 2023. Int J Mol Sci. 24: PMID: 37511316

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SA-2 Antibody (G-12) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-SA-2 Antibody (G-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SA-2 Antibody (G-12) Specifications

Alternate Product Name: STAG2

Product Clone Name: G-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 1174-1203

Epitope Target: SA-2

Epitope Species: human

Product Reactivity/Detection: SA-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for SA-2 Antibody (G-12): P: Blocking Peptide

The preferred SA-2 antibody is SA-2 Antibody (J-12): sc-81852.