SA-2 Antibody (J-12): sc-81852

SA-2 Antibody (J-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects SA-2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SA-2 (J-12) antibody is available in a non-conjugated form, providing flexibility for various experimental needs. SA-2, also known as STAG2, is a crucial 1,231 amino acid component of the cohesion complex, which plays a vital role in ensuring proper segregation of sister chromatids during cell division. SA-2 is localized in the nucleus, where SA-2 associates with chromatin and maintains genomic stability. Upon phosphorylation by polo-like kinase (Plk), SA-2 dissociates from chromatin, facilitating correct separation of chromosomes during anaphase. SA-2 has been shown to enhance tumor necrosis factor alpha (TNFα) activity, suggesting a potential role as a transcriptional regulator, which may have implications in cancer biology and therapeutic strategies. SA-2′s interaction with Rad21 and other components of the cohesion complex underscores SA-2′s importance in cell cycle regulation and chromosomal integrity.

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SA-2 Antibody (J-12) References:

1. Characterization of vertebrate cohesin complexes and their regulation in prophase.  |  Sumara, I., et al. 2000. J Cell Biol. 151: 749-62. PMID: 11076961
2. STAG2 and Rad21 mammalian mitotic cohesins are implicated in meiosis.  |  Prieto, I., et al. 2002. EMBO Rep. 3: 543-50. PMID: 12034751
3. Evidence of a transcriptional co-activator function of cohesin STAG/SA/Scc3.  |  Lara-Pezzi, E., et al. 2004. J Biol Chem. 279: 6553-9. PMID: 14660624
4. Dissociation of cohesin from chromosome arms and loss of arm cohesion during early mitosis depends on phosphorylation of SA2.  |  Hauf, S., et al. 2005. PLoS Biol. 3: e69. PMID: 15737063
5. Shugoshin prevents dissociation of cohesin from centromeres during mitosis in vertebrate cells.  |  McGuinness, BE., et al. 2005. PLoS Biol. 3: e86. PMID: 15737064
6. Cohesion proteins are present in centromere protein bodies associated with avian lampbrush chromosomes.  |  Krasikova, A., et al. 2005. Chromosome Res. 13: 675-85. PMID: 16235117
7. Genomic landscape of Ewing sarcoma defines an aggressive subtype with co-association of STAG2 and TP53 mutations.  |  Tirode, F., et al. 2014. Cancer Discov. 4: 1342-53. PMID: 25223734
8. The role of STAG2 in bladder cancer.  |  Aquila, L., et al. 2018. Pharmacol Res. 131: 143-149. PMID: 29501732
9. Cohesin Members Stag1 and Stag2 Display Distinct Roles in Chromatin Accessibility and Topological Control of HSC Self-Renewal and Differentiation.  |  Viny, AD., et al. 2019. Cell Stem Cell. 25: 682-696.e8. PMID: 31495782
10. STAG2 loss rewires oncogenic and developmental programs to promote metastasis in Ewing sarcoma.  |  Adane, B., et al. 2021. Cancer Cell. 39: 827-844.e10. PMID: 34129824
11. STAG2 regulates interferon signaling in melanoma via enhancer loop reprogramming.  |  Chu, Z., et al. 2022. Nat Commun. 13: 1859. PMID: 35388001
12. STAG2 Regulates Homologous Recombination Repair and Sensitivity to ATM Inhibition.  |  Zhou, J., et al. 2023. Adv Sci (Weinh). 10: e2302494. PMID: 37985839

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SA-2 Antibody (J-12) has 41 citations in a variety of scientific publications.

Primary antibodies like Anti-SA-2 Antibody (J-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SA-2 Antibody (J-12) Specifications

Alternate Product Name: SA-2

Product Clone Name: J-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1130-1232

Epitope Target: SA-2

Epitope Species: human

Product Reactivity/Detection: SA-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 41 citations in various scientific publications

Available Conjugates/Forms for SA-2 Antibody (J-12): N/A