SA-2 Antibody (J-12) recommended for detection of SA-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

SA-2 Antibody (J-12): sc-81852

SA-2 Antibody (J-12) is rated 4.4 out of 5 by 7.
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Datasheets

    • SA-2 Antibody (J-12) is a mouse monoclonal IgG1 (kappa light chain) provided at 100 µg/ml
    • raised against recombinant SA-2 of human origin
    • recommended for detection of SA-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of SA-2 (J-12): sc-81852.
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Hi Sir/ Mrs, I am interested in STAG2 antibody, but I do need to know the sequence of the antigen you used to make this antibody because I need it to do matching with STAG2 of dog. I need to know if this human STAG2 is suitable for dog...Thanks a lot.

Asked by: IJLafta
Thank you for your question. The specific antigen used to make this antibody, SA-2 (J-12): sc-81852, is considered proprietary. Please contact our Technical Service Department and we are happy to perform the sequence analysis for you to predict whether or not the antibody may cross-react with STAG2 of dog origin. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2018-01-16

I am wondering if you have STAG2 (SA-2) monoclonal antibody that is suitable to be used in IHC of dog tissues.   Thanks D. Inam Lafta

Asked by: I J L
Thank you for your question. This monoclonal antibody, SA-2 Antibody (J-12): sc-81852, has not been tested for reactivity with dog, and we are not aware of any publications citing use of the antibody in this species. Unfortunately, none of our SA-2 antibodies have been tested in, or are currently recommended for detection of the protein in dog tissues.
Answered by: Technical Support
Date published: 2017-09-28

Do you recommend using this mouse monoclonal SA-2 antibody for staining mouse tissue?

Asked by: cjMara
Thank you for your question. Yes, SA-2 Antibody (J-12): sc-81852 can be used for IF and IHC(P) on mouse tissue. We recommend using our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP): sc-516102 for specific detection of the primary antibody with minimal background.
Answered by: Technical Support 13
Date published: 2017-01-09
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Rated 5 out of 5 by from good quality antibody Gives good and specific signal for both unmodified and mono-ubiquitinated SA-2 on WB (use no less than 1:800).
Date published: 2017-06-03
Rated 5 out of 5 by from Great product Strong, specific western data; also nice product for IHC and IF staining applications
Date published: 2017-01-26
Rated 5 out of 5 by from Versatile Antibody This antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining.
Date published: 2017-01-09
Rated 4 out of 5 by from Nice band seen in WB using HeLa nuclear extract Nice band seen in WB using HeLa nuclear extract. -SCBT QC
Date published: 2015-07-14
Rated 4 out of 5 by from Publishable data in WB using human H4 cells Publishable data in WB using human H4 cells -SCBT Publication Review
Date published: 2015-06-25
Rated 4 out of 5 by from Various publishable data in IHC(P) using Various publishable data in IHC(P) using human H4 cells and bladder tumors. -SCBT Publication Review
Date published: 2015-06-10
Rated 4 out of 5 by from Robust immunoperoxidase nuclear staining Robust immunoperoxidase nuclear staining in formalin-fixed, paraffin-embedded human lymph node tissue. -SCBT QC
Date published: 2015-03-18
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