S-100 β chain Antibody (C-3): sc-393919

S-100 β chain Antibody (C-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects S-100 β chain protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). S-100 β chain (C-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. S-100 β chain protein plays a crucial role in regulating cellular processes, including cell cycle progression and differentiation, which are vital for maintaining normal cellular function and tissue homeostasis. S-100 proteins are implicated in activating calcium-induced calcium release, inhibiting microtubule assembly, and modulating protein kinase C-mediated phosphorylation, highlighting their importance in signal transduction pathways. S-100 family consists of two subunits, S-100 α and S-100 β, which share approximately 60% sequence identity and can form various dimeric combinations, including two α chains, two β chains, or one of each. S-100 β chain is predominantly localized in cytoplasm and nuclei of astrocytes, Schwann cells, and certain tumors, such as ependymomas and astrogliomas, and is also found in benign nevi and malignant melanocytic tumors, as well as in Langerhans cells in skin. S-100 proteins, along with calbindin and parvalbumin, are particularly significant in neural tissues, while S-100 α and β are also present in various other tissues, including intestine and kidney, underscoring their diverse biological roles.

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S-100 β chain Antibody (C-3) References:

1. [Pathophysiologic aspects of S-100beta protein: a new biological marker of brain pathology].  |  Beaudeux, J., et al. 1999. Ann Biol Clin (Paris). 57: 261-72. PMID: 10377476
2. Isolation of a rat S100 alpha cDNA and distribution of its mRNA in rat tissues.  |  Zimmer, DB., et al. 1991. Brain Res Bull. 27: 157-62. PMID: 1742602
3. Calcium-binding proteins of the EF-type.  |  Heizmann, CW. 1988. J Cardiovasc Pharmacol. 12 Suppl 5: S30-7. PMID: 2469875
4. S100B protein concentration measurement according to two different immunoassays.  |  Feriel, J., et al. 2015. Clin Chem Lab Med. 53: e169-71. PMID: 25719325
5. Serum Neuron-specific Enolase and S100 Calcium-binding Protein B in Pediatric Diabetic Ketoacidosis.  |  Elshorbagy, HH., et al. 2019. J Clin Res Pediatr Endocrinol. 11: 374-387. PMID: 31067852
6. S100B and its association with HIV-associated neurocognitive disorders.  |  Limpruttidham, N., et al. 2019. J Neurovirol. 25: 899-900. PMID: 31278533
7. Developmental and functional studies of parvalbumin and calbindin D28K in hypothalamic neurons grown in serum-free medium.  |  Pfyffer, GE., et al. 1987. J Neurochem. 49: 442-51. PMID: 3298546
8. Developmental appearance of the Ca2+-binding proteins parvalbumin, calbindin D-28K, S-100 proteins and calmodulin during testicular development in the rat.  |  Kägi, U., et al. 1988. Cell Tissue Res. 252: 359-65. PMID: 3383215
9. Increase of skeletal muscle relaxation speed by direct injection of parvalbumin cDNA.  |  Müntener, M., et al. 1995. Proc Natl Acad Sci U S A. 92: 6504-8. PMID: 7604022
10. S100 protein expression in subpopulations of neurons of rat brain.  |  Rickmann, M. and Wolff, JR. 1995. Neuroscience. 67: 977-91. PMID: 7675218
11. Retinoic acid regulates the expression of the calcium binding protein, calbindin-D28K.  |  Wang, YZ. and Christakos, S. 1995. Mol Endocrinol. 9: 1510-21. PMID: 8584029
12. A novel calcium-binding protein in amniotic fluid, CAAF1: its molecular cloning and tissue distribution.  |  Hitomi, J., et al. 1996. J Cell Sci. 109 (Pt 4): 805-15. PMID: 8718672

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-S-100 β chain Antibody (C-3) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-S-100 β chain Antibody (C-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

S-100 β chain Antibody (C-3) Specifications

Alternate Product Name: S100 protein B (S100B)

Product Clone Name: C-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 34-48

Epitope Target: S-100 β chain

Epitope Species: human

Product Reactivity/Detection: S-100 β chain

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for S-100 β chain Antibody (C-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred S-100 α/β chain antibody is S-100 α/β chain Antibody (B32.1): sc-58839.