RyR-1 Antibody (XA7): sc-65640

RyR-1 Antibody (XA7) is a mouse monoclonal IgM antibody that detects RyR-1 protein of mouse, rat, rabbit, and canine origin by western blotting (WB) and immunoprecipitation (IP). Anti-RyR-1 antibody (XA7) is available as a non-conjugated format, specifically targeting ryanodine receptor 1, which plays a crucial role in muscle contraction by mediating calcium release from the sarcoplasmic reticulum. RyR-1 (XA7) monoclonal antibody recognizes RyR-1 protein primarily located in the sarcoplasmic reticulum of skeletal muscle cells, where RyR-1 functions as a calcium release channel. This localization allows for rapid release of calcium ions in response to electrical signals, facilitating excitation-contraction coupling essential for muscle function. Disruptions in RyR-1 function can lead to severe muscle disorders, including malignant hyperthermia and central core disease, highlighting RyR-1′s importance in maintaining normal muscle physiology. The RyR family, including RyR-1, RyR-2, and RyR-3, is integral to calcium signaling in various tissues, with RyR-1 being specifically associated with skeletal muscle. Understanding RyR-1′s role and location is essential for developing therapeutic strategies for muscle-related diseases.

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RyR-1 Antibody (XA7) References:

1. A genetic model for the study of abnormal nerve-muscle interactions at the level of excitation-contraction coupling: the mutation muscular dysgenesis.  |  Pinçon-Raymond, M., et al. 1990. J Physiol (Paris). 84: 82-7. PMID: 2193149
2. Binding sites of monoclonal antibodies and dihydropyridine receptor alpha 1 subunit cytoplasmic II-III loop on skeletal muscle triadin fusion peptides.  |  Fan, H., et al. 1995. Biochemistry. 34: 14893-901. PMID: 7578101
3. Phosphorylation of dihydropyridine receptor II-III loop peptide regulates skeletal muscle calcium release channel function. Evidence for an essential role of the beta-OH group of Ser687.  |  Lu, X., et al. 1995. J Biol Chem. 270: 18459-64. PMID: 7629172
4. Formation of triads without the dihydropyridine receptor alpha subunits in cell lines from dysgenic skeletal muscle.  |  Powell, JA., et al. 1996. J Cell Biol. 134: 375-87. PMID: 8707823
5. Formation of junctions involved in excitation-contraction coupling in skeletal and cardiac muscle.  |  Flucher, BE. and Franzini-Armstrong, C. 1996. Proc Natl Acad Sci U S A. 93: 8101-6. PMID: 8755610
6. Functional nonequality of the cardiac and skeletal ryanodine receptors.  |  Nakai, J., et al. 1997. Proc Natl Acad Sci U S A. 94: 1019-22. PMID: 9023375
7. A carboxy-terminal peptide of the alpha 1-subunit of the dihydropyridine receptor inhibits Ca(2+)-release channels.  |  Slavik, KJ., et al. 1997. Am J Physiol. 272: C1475-81. PMID: 9176137
8. Ryanodine receptors of striated muscles: a complex channel capable of multiple interactions.  |  Franzini-Armstrong, C. and Protasi, F. 1997. Physiol Rev. 77: 699-729. PMID: 9234963

The preferred RyR antibody is RyR Antibody (F-1): sc-376507.