Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RXRβ CRISPR/Cas9 KO Plasmid (m): sc-422768
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RXRβ CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RXRβ genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RXRβ Antibody (11-13): sc-742
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RXRβ CRISPR/Cas9 KO Plasmid (m) | sc-422768 | 20 µg | $397.00 |
Overview
Rxrb encodes retinoid X receptor beta (RXRβ), a ligand-activated nuclear receptor that forms heterodimers with multiple partners including PPARs, LXRs, FXR, VDR, and RARs to regulate transcriptional programs controlling lipid and glucose metabolism, cell differentiation, and inflammatory signaling. RXRβ integrates retinoid and lipid-derived cues to modulate chromatin-associated transcription at response elements, influencing mitochondrial function and oxidative balance in metabolically active tissues. In mouse models, altered Rxrb activity has been linked to dysregulated metabolic homeostasis and immune modulation, making it relevant to studies of steatosis, insulin sensitivity, and inflammatory disease mechanisms. Its central role in nuclear receptor crosstalk also positions RXRβ as a node for dissecting pathway interactions in development and endocrine signaling.
RXRβ CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Rxrb gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Rxrb together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Rxrb open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RXRβ protein expression.
This CRISPR knockout system enables efficient generation of Rxrb-deficient cell models for investigation of RXRβ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Rxrb exon(s) critical for RXRβ function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Rxrb genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RXRβ CRISPR/Cas9 KO Plasmid (m) and RXRβ CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Rxrb locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RXRβ HDR Plasmid (m) and RXRβ HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Rxrb homology arms to support homology-directed repair at defined Rxrb target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.