RUNX2 Antibody (F-2): sc-390351
- RUNX2 Antibody (F-2) is a mouse monoclonal IgG1 κ RUNX2 antibody, cited in 141 publications, provided at 200 µg/ml
- raised against amino acids 294-363 of RUNX2 of mouse origin
- RUNX2 Antibody (F-2) is recommended for detection of RUNX2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, bovine and porcine
- Anti-RUNX2 Antibody (F-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-390351 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for RUNX2 Antibody (F-2) for WB and IHC(P) applications. This reagent is now offered in a bundle with RUNX2 Antibody (F-2) (see ordering information below).
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RUNX2 Antibody (F-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects RUNX2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-RUNX2 antibody (F-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RUNX2 plays a critical role in skeletal development and mineralization, functioning as a transcription factor that regulates the expression of genes essential for osteoblast differentiation and bone formation. RUNX2 stimulates the differentiation of mesenchymal stem cells into osteoblasts for proper skeletal development and promotes chondrocyte hypertrophy, which is crucial for endochondral ossification. RUNX2 drives endothelial cell migration and vascular invasion during bone development, highlighting RUNX2′s importance in both skeletal and vascular systems. Dysregulation of RUNX2 expression can lead to various skeletal disorders, including cleidocranial dysplasia syndrome, characterized by skeletal abnormalities such as clavicular hypoplasia and supernumerary teeth. RUNX2 (F-2) antibody′s versatility in multiple applications makes this reagent an invaluable tool for researchers studying bone biology and related pathologies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RUNX2 Antibody (F-2) References:
- Role of RUNX2 in Breast Carcinogenesis. | Wysokinski, D., et al. 2015. Int J Mol Sci. 16: 20969-93. PMID: 26404249
- Cleidocranial dysplasia and RUNX2-clinical phenotype-genotype correlation. | Jaruga, A., et al. 2016. Clin Genet. 90: 393-402. PMID: 27272193
- Regulation of Runx2 by MicroRNAs in osteoblast differentiation. | Narayanan, A., et al. 2019. Life Sci. 232: 116676. PMID: 31340165
- Molecular Mechanism of Runx2-Dependent Bone Development. | Komori, T. 2020. Mol Cells. 43: 168-175. PMID: 31896233
- RUNX2 Regulates Osteoblast Differentiation via the BMP4 Signaling Pathway. | Liu, DD., et al. 2022. J Dent Res. 101: 1227-1237. PMID: 35619284
- Runx2 regulates chromatin accessibility to direct the osteoblast program at neonatal stages. | Hojo, H., et al. 2022. Cell Rep. 40: 111315. PMID: 36070691
- Runx2 and Runx3 differentially regulate articular chondrocytes during surgically induced osteoarthritis development. | Nagata, K., et al. 2022. Nat Commun. 13: 6187. PMID: 36261443
- Carbonylation of Runx2 at K176 by 4-Hydroxynonenal Accelerates Vascular Calcification. | Zhai, X., et al. 2024. Circulation. 149: 1752-1769. PMID: 38348663
- FOXO1 regulates RUNX2 ubiquitination through SMURF2 in calcific aortic valve disease. | Jiang, C., et al. 2024. Redox Biol. 73: 103215. PMID: 38810422
- RUNX2 as a Prognostic Factor in Human Cancers. | Toner, J., et al. 2024. Crit Rev Eukaryot Gene Expr. 34: 51-66. PMID: 39072409
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RUNX2 Antibody (F-2) | sc-390351 | 200 µg/ml | $322.00 | |||
RUNX2 Antibody (F-2): m-IgG Fc BP-HRP Bundle | sc-526149 | 200 µg Ab; 10 µg BP | $361.00 | |||
RUNX2 Antibody (F-2) X | sc-390351 X | 200 µg/0.1 ml | $322.00 | |||
RUNX2 Antibody (F-2) AC | sc-390351 AC | 500 µg/ml, 25% agarose | $424.00 | |||
RUNX2 Antibody (F-2) HRP | sc-390351 HRP | 200 µg/ml | $322.00 | |||
RUNX2 Antibody (F-2) FITC | sc-390351 FITC | 200 µg/ml | $336.00 | |||
RUNX2 Antibody (F-2) PE | sc-390351 PE | 200 µg/ml | $349.00 | |||
RUNX2 Antibody (F-2) Alexa Fluor® 488 | sc-390351 AF488 | 200 µg/ml | $364.00 | |||
RUNX2 Antibody (F-2) Alexa Fluor® 546 | sc-390351 AF546 | 200 µg/ml | $364.00 | |||
RUNX2 Antibody (F-2) Alexa Fluor® 594 | sc-390351 AF594 | 200 µg/ml | $364.00 | |||
RUNX2 Antibody (F-2) Alexa Fluor® 647 | sc-390351 AF647 | 200 µg/ml | $364.00 | |||
RUNX2 Antibody (F-2) Alexa Fluor® 680 | sc-390351 AF680 | 200 µg/ml | $364.00 | |||
RUNX2 Antibody (F-2) Alexa Fluor® 790 | sc-390351 AF790 | 200 µg/ml | $364.00 |