Rsk-1 Antibody (A-10) recommended for detection of Rsk-1 of mouse, rat, human and mink origin by WB, IP, IF and ELISA

Rsk-1 Antibody (A-10): sc-393147

Rsk-1 Antibody (A-10) is rated 4.7 out of 5 by 3.
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Datasheets

    • Anti-Rsk-1 Antibody (A-10) is a mouse monoclonal IgG1 (kappa light chain) Rsk-1 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 707-735 at the C-terminus of Rsk-1 of human origin
    • Anti-Rsk-1 Antibody (A-10) is recommended for detection of Rsk-1 of mouse, rat, human and mink origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
    • Anti-Rsk-1 Antibody (A-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Rsk-1 (A-10): sc-393147.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Rsk-1 Antibody (A-10) for WB applications. This reagent is now offered in a bundle with Rsk-1 Antibody (A-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Has sc-393147 been validated for cross-reactivity with RSK2 and other subtypes?

Asked by: bona
Thank you for your question. This monoclonal antibody is specific for an epitope for Rsk-1, and is not expected to cross-react with other Rsk family members. Potential cross-reactivity has not been evaluated experimentally.
Answered by: Technical Support
Date published: 2019-07-26

What application is the blocking peptide sc-393147 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from great antibody I have used the antibody for many years. It is great. I also have tried RSK1 from many other companies. the results were not good. I give 5 stars for this antibody
Date published: 2016-12-12
Rated 4 out of 5 by from Potent and fairly specific antibody This monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was medium detection of the ~90 kDa target protein in a HeLa/Jurkat cell lysate mix, but not detected in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested. A ~26 kDa strong cross-reactive protein was evident in the HeLa/Jurkat cell lysate mix.
Date published: 2016-11-10
Rated 5 out of 5 by from Produced positive Western blot data of Rsk Produced positive Western blot data of Rsk-1 expression in HeLa, K-562, Mv 1 Lu, A-431 and MCF7 whole cell lysates. -SCBT QC
Date published: 2014-02-08
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