RRP12 Antibody (A-3): sc-398593

RRP12 Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects RRP12 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RRP12 (A-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RRP12 protein, a 1,297 amino acid single-pass membrane protein, plays a crucial role in ribosomal assembly, a process essential for protein synthesis within cells. Located primarily in the nucleus and expressed in testis and ovary, RRP12 is part of the RRP12 family and is involved in nucleocytoplasmic translocation of pre-ribosomes, working alongside Dim2 to ensure proper ribosome production. This function is particularly important as RRP12 links ribosome biogenesis to regulation of other cellular processes during cell cycle progression, thereby influencing overall cellular health and function. RRP12 exists in two alternatively spliced isoforms and is encoded by a gene located on human chromosome 10q24.1, highlighting evolutionary significance and potential implications in various biological contexts.

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RRP12 Antibody (A-3) References:

1. The human ribosomal protein genes: sequencing and comparative analysis of 73 genes.  |  Yoshihama, M., et al. 2002. Genome Res. 12: 379-90. PMID: 11875025
2. A pre-ribosome-associated HEAT-repeat protein is required for export of both ribosomal subunits.  |  Oeffinger, M., et al. 2004. Genes Dev. 18: 196-209. PMID: 14729571
3. Separation of the Saccharomyces cerevisiae Paf1 complex from RNA polymerase II results in changes in its subnuclear localization.  |  Porter, SE., et al. 2005. Eukaryot Cell. 4: 209-20. PMID: 15643076
4. TOR regulates the subcellular distribution of DIM2, a KH domain protein required for cotranscriptional ribosome assembly and pre-40S ribosome export.  |  Vanrobays, E., et al. 2008. RNA. 14: 2061-73. PMID: 18755838
5. Tandem affinity purification combined with inducible shRNA expression as a tool to study the maturation of macromolecular assemblies.  |  Wyler, E., et al. 2011. RNA. 17: 189-200. PMID: 21097556
6. Ribosome synthesis-unrelated functions of the preribosomal factor Rrp12 in cell cycle progression and the DNA damage response.  |  Dosil, M. 2011. Mol Cell Biol. 31: 2422-38. PMID: 21482668
7. Rrp12 and the Exportin Crm1 participate in late assembly events in the nucleolus during 40S ribosomal subunit biogenesis.  |  Moriggi, G., et al. 2014. PLoS Genet. 10: e1004836. PMID: 25474739
8. RRP12 is a crucial nucleolar protein that regulates p53 activity in osteosarcoma cells.  |  Choi, YJ., et al. 2016. Tumour Biol. 37: 4351-8. PMID: 26499779
9. Validating RRP12 Expression and Its Prognostic Significance in HCC Based on Data Mining and Bioinformatics Methods.  |  Wei, C., et al. 2022. Front Oncol. 12: 812009. PMID: 35178347
10. Cms1 coordinates stepwise local 90S pre-ribosome assembly with timely snR83 release.  |  Lau, B., et al. 2022. Cell Rep. 41: 111684. PMID: 36417864
11. RRP12 suppresses cell migration and invasion in colorectal cancer cell via regulation of epithelial-mesenchymal transition.  |  An, G., et al. 2023. J Gastrointest Oncol. 14: 2111-2123. PMID: 37969827
12. A map of 75 human ribosomal protein genes.  |  Kenmochi, N., et al. 1998. Genome Res. 8: 509-23. PMID: 9582194

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RRP12 Antibody (A-3) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-RRP12 Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RRP12 Antibody (A-3) Specifications

Alternate Product Name: RRP12

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 538-576

Epitope Target: RRP12

Epitope Species: human

Product Reactivity/Detection: RRP12

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for RRP12 Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin