Rpp38 Antibody (A-2): sc-398112
- Rpp38 Antibody (A-2) is a mouse monoclonal IgG1 κ Rpp38 antibody provided at 200 µg/ml
- raised against amino acids 205-246 mapping near the C-terminus of Rpp38 of human origin
- Rpp38 Antibody (A-2) is recommended for detection of Rpp38 of human origin by WB, IP, IF and ELISA
- Anti-Rpp38 Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Rpp38 Antibody (A-2) for WB applications. These reagents are now offered in bundles with Rpp38 Antibody (A-2) (see ordering information below).
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Rpp38 Antibody (A-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Rpp38 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Rpp38 antibody (A-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Rpp38 is a crucial component of the ribonuclease P (RNase P) complex, which is essential for the maturation of precursor tRNA molecules by removing their 5-prime leader sequences. This enzymatic activity is vital for the proper functioning of tRNA, which plays a key role in protein synthesis. Additionally, Rpp38 is involved in the formation of the 5′ end of 5.8S rRNA through its association with ribonuclease MRP (RNase MRP), highlighting its importance in ribosomal RNA processing. Both RNase P and RNase MRP are localized in the nucleolus, where they contribute to the intricate processes of RNA maturation and modification. Notably, Rpp38 has been implicated as a Th autoantigen in systemic sclerosis, an autoimmune disease, underscoring its relevance in both cellular function and disease pathology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rpp38 Antibody (A-2) References:
- RNA-protein interactions in the human RNase MRP ribonucleoprotein complex. | Pluk, H., et al. 1999. RNA. 5: 512-24. PMID: 10199568
- Architecture and function of the human endonucleases RNase P and RNase MRP. | van Eenennaam, H., et al. 2000. IUBMB Life. 49: 265-72. PMID: 10995027
- Ribonuclease P: a ribonucleoprotein enzyme. | Kurz, JC. and Fierke, CA. 2000. Curr Opin Chem Biol. 4: 553-8. PMID: 11006544
- The road to RNase P. | Altman, S. 2000. Nat Struct Biol. 7: 827-8. PMID: 11017184
- Basic domains target protein subunits of the RNase MRP complex to the nucleolus independently of complex association. | van Eenennaam, H., et al. 2001. Mol Biol Cell. 12: 3680-9. PMID: 11694598
- RNase MRP/RNase P: a structure-function relation conserved in evolution? | Karwan, R. 1993. FEBS Lett. 319: 1-4. PMID: 7681014
- Autoantigenic properties of some protein subunits of catalytically active complexes of human ribonuclease P. | Jarrous, N., et al. 1998. RNA. 4: 407-17. PMID: 9630247
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rpp38 Antibody (A-2) | sc-398112 | 200 µg/ml | $322.00 | |||
Rpp38 Antibody (A-2): m-IgG Fc BP-HRP Bundle | sc-530693 | 200 µg Ab; 10 µg BP | $361.00 | |||
Rpp38 Antibody (A-2): m-IgGκ BP-HRP Bundle | sc-524219 | 200 µg Ab, 40 µg BP | $361.00 | |||
Rpp38 Antibody (A-2): m-IgG1 BP-HRP Bundle | sc-544202 | 200 µg Ab; 20 µg BP | $361.00 | |||
Rpp38 Antibody (A-2) AC | sc-398112 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Rpp38 Antibody (A-2) HRP | sc-398112 HRP | 200 µg/ml | $322.00 | |||
Rpp38 Antibody (A-2) FITC | sc-398112 FITC | 200 µg/ml | $336.00 | |||
Rpp38 Antibody (A-2) PE | sc-398112 PE | 200 µg/ml | $349.00 | |||
Rpp38 Antibody (A-2) Alexa Fluor® 488 | sc-398112 AF488 | 200 µg/ml | $364.00 | |||
Rpp38 Antibody (A-2) Alexa Fluor® 546 | sc-398112 AF546 | 200 µg/ml | $364.00 | |||
Rpp38 Antibody (A-2) Alexa Fluor® 594 | sc-398112 AF594 | 200 µg/ml | $364.00 | |||
Rpp38 Antibody (A-2) Alexa Fluor® 647 | sc-398112 AF647 | 200 µg/ml | $364.00 | |||
Rpp38 Antibody (A-2) Alexa Fluor® 680 | sc-398112 AF680 | 200 µg/ml | $364.00 | |||
Rpp38 Antibody (A-2) Alexa Fluor® 790 | sc-398112 AF790 | 200 µg/ml | $364.00 |