Rpp20 Antibody (D-6): sc-514903
- Rpp20 Antibody (D-6) is a mouse monoclonal IgM κ Rpp20 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 3-27 at the N-terminus of Rpp20 of human origin
- recommended for detection of Rpp20 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Rpp20 Antibody (D-6). This work is in progress.
QUICK LINKS
Rpp20 Antibody (D-6) is a mouse monoclonal IgM antibody that detects Rpp20 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Rpp20 antibody (D-6) is available as the non-conjugated form. Rpp20, also known as ribonuclease P protein subunit p20 or POP7, is a crucial component of the ribonuclease P and ribonuclease MRP complexes, which are essential for RNA processing in the cell. These complexes are primarily located in the nucleolus, where they play a vital role in the maturation of precursor tRNA molecules by removing their 5′ leader sequences, a process that is critical for the proper functioning of tRNA in protein synthesis. Rpp20, consisting of 140 amino acids, belongs to the histone-like Alba family and is encoded by a gene located on human chromosome 7q22.1 and mouse chromosome 5 G2. The interaction of Rpp20 with other protein subunits, such as POP1 and POP5, highlights its importance in maintaining the structural integrity and functionality of the ribonucleoprotein complexes, thereby ensuring efficient RNA processing and cellular function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Rpp20 Antibody (D-6) References:
- Protein-protein interactions with subunits of human nuclear RNase P. | Jiang, T. and Altman, S. 2001. Proc Natl Acad Sci U S A. 98: 920-5. PMID: 11158571
- Rpp20 interacts with SMN and is re-distributed into SMN granules in response to stress. | Hua, Y. and Zhou, J. 2004. Biochem Biophys Res Commun. 314: 268-76. PMID: 14715275
- Mutual interactions between subunits of the human RNase MRP ribonucleoprotein complex. | Welting, TJ., et al. 2004. Nucleic Acids Res. 32: 2138-46. PMID: 15096576
- Heterodimerization of the human RNase P/MRP subunits Rpp20 and Rpp25 is a prerequisite for interaction with the P3 arm of RNase MRP RNA. | Hands-Taylor, KL., et al. 2010. Nucleic Acids Res. 38: 4052-66. PMID: 20215441
- Structure-function analysis in nuclear RNase P RNA. | Engelke, DR., et al. Mol Biol Rep. 22: 157-60. PMID: 8901504
- Rpp2, an essential protein subunit of nuclear RNase P, is required for processing of precursor tRNAs and 35S precursor rRNA in Saccharomyces cerevisiae. | Stolc, V., et al. 1998. Proc Natl Acad Sci U S A. 95: 6716-21. PMID: 9618478
- Autoantigenic properties of some protein subunits of catalytically active complexes of human ribonuclease P. | Jarrous, N., et al. 1998. RNA. 4: 407-17. PMID: 9630247
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Rpp20 Antibody (D-6) | sc-514903 | 200 µg/ml | $316.00 | |||
Rpp20 (D-6) Neutralizing Peptide | sc-514903 P | 100 µg/0.5 ml | $68.00 |