RPIA Antibody (D-5): sc-515328
- RPIA Antibody (D-5) is a mouse monoclonal IgG1 κ RPIA antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 177-311 mapping at the C-terminus of RPIA of human origin
- RPIA Antibody (D-5) is recommended for detection of RPIA of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-RPIA Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RPIA Antibody (D-5) for WB applications. These reagents are now offered in bundles with RPIA Antibody (D-5) (see ordering information below).
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SEE ALSO...
RPIA Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects RPIA protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RPIA (D-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RPIA protein, also known as ribose 5-phosphate isomerase A, is a crucial enzyme consisting of 311 amino acids that plays a vital role in the pentose-phosphate pathway by catalyzing the conversion of ribose-5-phosphate to ribulose-5-phosphate. This enzymatic function is essential for carbohydrate metabolism, as RPIA helps generate ribose-5-phosphate, a precursor for nucleotide synthesis, thereby influencing cellular proliferation and energy metabolism. RPIA is a member of the ribose 5-phosphate isomerase family and is encoded by a gene located on human chromosome 2p11.2. Mutations in the RPIA gene can lead to ribose 5-phosphate isomerase deficiency (RPID), a rare disorder associated with neurological complications such as leukoencephalopathy and peripheral neuropathy. Additionally, chromosome 2 is linked to several other genetic disorders, including Harlequin ichthyosis, sitosterolemia, and Alström syndrome, highlighting RPIA′s importance in both metabolic pathways and disease contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RPIA Antibody (D-5) References:
- An ATP-binding cassette gene (ABCG5) from the ABCG (White) gene subfamily maps to human chromosome 2p21 in the region of the Sitosterolemia locus. | Shulenin, S., et al. 2001. Cytogenet Cell Genet. 92: 204-8. PMID: 11435688
- Mutation of ALMS1, a large gene with a tandem repeat encoding 47 amino acids, causes Alström syndrome. | Hearn, T., et al. 2002. Nat Genet. 31: 79-83. PMID: 11941370
- Ribose-5-phosphate isomerase deficiency: new inborn error in the pentose phosphate pathway associated with a slowly progressive leukoencephalopathy. | Huck, JH., et al. 2004. Am J Hum Genet. 74: 745-51. PMID: 14988808
- Mutations in ABCA12 underlie the severe congenital skin disease harlequin ichthyosis. | Kelsell, DP., et al. 2005. Am J Hum Genet. 76: 794-803. PMID: 15756637
- The biochemistry, metabolism and inherited defects of the pentose phosphate pathway: a review. | Wamelink, MM., et al. 2008. J Inherit Metab Dis. 31: 703-17. PMID: 18987987
- The unexplored potential of the pentose phosphate pathway in health and disease. | Berry, GT. 2008. J Inherit Metab Dis. 31: 661. PMID: 19015949
- The pentose phosphate pathway in the endoplasmic reticulum. | Bublitz, C. and Steavenson, S. 1988. J Biol Chem. 263: 12849-53. PMID: 2843500
- Biochemical genetics of the pentose phosphate cycle: human ribose 5-phosphate isomerase (RPI) and ribulose 5-phosphate 3-epimerase (RPE). | Spencer, N. and Hopkinson, DA. 1980. Ann Hum Genet. 43: 335-42. PMID: 7396409
- The ribose 5-phosphate isomerase-encoding gene is located immediately downstream from that encoding murine immunoglobulin kappa. | Apel, TW., et al. 1995. Gene. 156: 191-7. PMID: 7758956
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RPIA Antibody (D-5) | sc-515328 | 200 µg/ml | $316.00 | |||
RPIA Antibody (D-5): m-IgG Fc BP-HRP Bundle | sc-531339 | 200 µg Ab; 10 µg BP | $354.00 | |||
RPIA Antibody (D-5): m-IgGκ BP-HRP Bundle | sc-525004 | 200 µg Ab, 40 µg BP | $354.00 | |||
RPIA Antibody (D-5) AC | sc-515328 AC | 500 µg/ml, 25% agarose | $416.00 | |||
RPIA Antibody (D-5) HRP | sc-515328 HRP | 200 µg/ml | $316.00 | |||
RPIA Antibody (D-5) FITC | sc-515328 FITC | 200 µg/ml | $330.00 | |||
RPIA Antibody (D-5) PE | sc-515328 PE | 200 µg/ml | $343.00 | |||
RPIA Antibody (D-5) Alexa Fluor® 488 | sc-515328 AF488 | 200 µg/ml | $357.00 | |||
RPIA Antibody (D-5) Alexa Fluor® 546 | sc-515328 AF546 | 200 µg/ml | $357.00 | |||
RPIA Antibody (D-5) Alexa Fluor® 594 | sc-515328 AF594 | 200 µg/ml | $357.00 | |||
RPIA Antibody (D-5) Alexa Fluor® 647 | sc-515328 AF647 | 200 µg/ml | $357.00 | |||
RPIA Antibody (D-5) Alexa Fluor® 680 | sc-515328 AF680 | 200 µg/ml | $357.00 | |||
RPIA Antibody (D-5) Alexa Fluor® 790 | sc-515328 AF790 | 200 µg/ml | $357.00 |