RPA135 Antibody (4H6): sc-293272

RPA135 Antibody (4H6) is a mouse monoclonal IgG2a kappa light chain antibody that detects RPA135 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-RPA135 antibody (4H6) is available as the non-conjugated format. RPA135 protein plays a crucial role as a subunit of RNA polymerase I (Pol I), which is essential for the transcription of ribosomal RNA genes, thereby facilitating ribosomal biogenesis. Located primarily in the nucleolus, RPA135 interacts with other Pol I subunits, such as RPA16 and RPA40, to form a functional transcription complex. Assembly of these subunits is tightly regulated by various physiological stimuli, indicating that multiple signaling pathways are involved in the regulation of Pol I transcription. Notably, RPA40 is not only vital for the integrity of the Pol I complex but also serves as an essential component of RNA polymerase III, highlighting the interconnectedness of these transcriptional machinery components. RPA135 (4H6) antibody effectively detects this protein across different species, making RPA135 (4H6) monoclonal antibody a valuable tool for researchers studying ribosomal RNA transcription and its regulatory mechanisms.

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RPA135 Antibody (4H6) References:

1. Human Nopp140, which interacts with RNA polymerase I: implications for rRNA gene transcription and nucleolar structural organization.  |  Chen, HK., et al. 1999. Mol Cell Biol. 19: 8536-46. PMID: 10567578
2. Brain RNA polymerase and nucleolar structure in perinatal asphyxia of the rat.  |  Mosgoeller, W., et al. 2000. Exp Neurol. 161: 174-82. PMID: 10683283
3. An approach for isolation of mutants defective in 35S ribosomal RNA synthesis in Saccharomyces cerevisiae.  |  Nogi, Y., et al. 1991. Proc Natl Acad Sci U S A. 88: 7026-30. PMID: 1871118
4. Ribosomal RNA Transcription Machineries in Intestinal Protozoan Parasites: A Bioinformatic Analysis.  |  Lagunas-Rangel, FA. 2022. Acta Parasitol. 67: 1788-1799. PMID: 36028726
5. Expression of RNA polymerase I catalytic core is influenced by RPA12.  |  Ford, BL., et al. 2023. PLoS One. 18: e0285660. PMID: 37167337
6. ZBTB34 is a hepatocellular carcinoma-associated protein with a monopartite nuclear localization signal.  |  Liu, Z., et al. 2023. Aging (Albany NY). 15: 8487-8500. PMID: 37650557
7. Low baseline ribosome-related gene expression and resistance training-induced declines in ribosome-related gene expression are associated with skeletal muscle hypertrophy in young men and women.  |  Brown, A., et al. 2024. J Cell Physiol. 239: e31182. PMID: 38214457
8. Honey Targets Ribosome Biogenesis Components to Suppress the Growth of Human Pancreatic Cancer Cells.  |  Bangash, AA., et al. 2024. Cancers (Basel). 16: PMID: 39410048
9. Mouse RNA polymerase I 16-kDa subunit able to associate with 40-kDa subunit is a homolog of yeast AC19 subunit of RNA polymerases I and III.  |  Yao, Y., et al. 1996. J Biol Chem. 271: 32881-5. PMID: 8955128
10. Molecular cloning and characterization of the cDNA encoding the largest subunit of mouse RNA polymerase I.  |  Seither, P., et al. 1997. Mol Gen Genet. 255: 180-6. PMID: 9236775
11. Deficient transcription of subunit RPA 40 of RNA polymerase I and III in heart of rats with neonatal asphyxia.  |  Hoeger, H., et al. 1998. Life Sci. 62: 275-82. PMID: 9450498
12. Regulation of mammalian ribosomal gene transcription by RNA polymerase I.  |  Grummt, I. 1999. Prog Nucleic Acid Res Mol Biol. 62: 109-54. PMID: 9932453

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RPA135 Antibody (4H6) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-RPA135 Antibody (4H6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RPA135 Antibody (4H6) Specifications

Alternate Product Name: RPA135

Product Clone Name: 4H6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 963-1072

Epitope Target: RPA135

Epitope Species: human

Product Reactivity/Detection: RPA135

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for RPA135 Antibody (4H6): N/A