RPA 70 kDa subunit Antibody (H-7): sc-48425

RPA 70 kDa subunit Antibody (H-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects the RPA 70 kDa subunit protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). RPA 70 kDa subunit (H-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RPA 70 kDa subunit is a critical component of the replication protein A complex, which is essential for DNA replication, repair, and recombination processes. RPA 70 kDa subunit′s ability to bind single-stranded DNA (ssDNA) is vital for maintaining genomic stability, as RPA 70 kDa subunit protects ssDNA from degradation and facilitates the recruitment of other proteins involved in DNA metabolism. RPA 70 kDa subunit′s interaction with ssDNA is characterized by a unique binding mechanism that involves a conserved four cysteine-type zinc-finger motif, which plays a crucial role in stabilizing the RPA-ssDNA complex in a redox-dependent manner. This stabilization is particularly important during the early stages of nucleotide excision repair, where RPA 70 kDa subunit acts as a damage-recognition protein, ensuring that DNA lesions are accurately identified and repaired. RPA 70 kDa subunit′s interactions with various cellular and viral proteins underscore RPA 70 kDa subunit′s significance in cellular processes, making RPA 70 kDa subunit a valuable target for research in DNA biology and therapeutic applications.

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RPA 70 kDa subunit Antibody (H-7) References:

1. Replication protein A interactions with DNA. 2. Characterization of double-stranded DNA-binding/helix-destabilization activities and the role of the zinc-finger domain in DNA interactions.  |  Lao, Y., et al. 1999. Biochemistry. 38: 3974-84. PMID: 10194309
2. Cryptosporidium parvum possesses a short-type replication protein A large subunit that differs from its host.  |  Zhu, G., et al. 1999. FEMS Microbiol Lett. 176: 367-72. PMID: 10427719
3. Photocrosslinking locates a binding site for the large subunit of human replication protein A to the damaged strand of cisplatin-modified DNA.  |  Schweizer, U., et al. 1999. Nucleic Acids Res. 27: 3183-9. PMID: 10454616
4. Replication protein A physically interacts with the Bloom's syndrome protein and stimulates its helicase activity.  |  Brosh, RM., et al. 2000. J Biol Chem. 275: 23500-8. PMID: 10825162
5. The 32-kilodalton subunit of replication protein A interacts with menin, the product of the MEN1 tumor suppressor gene.  |  Sukhodolets, KE., et al. 2003. Mol Cell Biol. 23: 493-509. PMID: 12509449
6. Replication protein A interactions with DNA: differential binding of the core domains and analysis of the DNA interaction surface.  |  Wyka, IM., et al. 2003. Biochemistry. 42: 12909-18. PMID: 14596605
7. Recruitment of replication protein A by the papillomavirus E1 protein and modulation by single-stranded DNA.  |  Loo, YM. and Melendy, T. 2004. J Virol. 78: 1605-15. PMID: 14747526
8. Regulation of DNA repair through deSUMOylation and SUMOylation of replication protein A complex.  |  Dou, H., et al. 2010. Mol Cell. 39: 333-45. PMID: 20705237
9. Role of the 70-kDa subunit of human replication protein A (I). Single-stranded dna binding activity, but not polymerase stimulatory activity, is required for DNA replication.  |  Kim, DK., et al. 1996. J Biol Chem. 271: 15124-9. PMID: 8663111
10. Role of protein-protein interactions in the function of replication protein A (RPA): RPA modulates the activity of DNA polymerase alpha by multiple mechanisms.  |  Braun, KA., et al. 1997. Biochemistry. 36: 8443-54. PMID: 9214288

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RPA 70 kDa subunit Antibody (H-7) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-RPA 70 kDa subunit Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RPA 70 kDa subunit Antibody (H-7) Specifications

Alternate Product Name: RPA70

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 317-616

Epitope Target: RPA 70 kDa subunit

Epitope Species: human

Product Reactivity/Detection: RPA 70 kDa subunit

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for RPA 70 kDa subunit Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred RPA 70 kDa subunit antibody is RPA 70 kDa subunit Antibody (B-6): sc-28304.