RPA 70 kDa subunit Antibody (B-6): sc-28304

RPA 70 kDa subunit Antibody (B-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects RPA 70 kDa subunit of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). RPA 70 kDa subunit Antibody (B-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RPA 70 kDa subunit is a critical component of the replication protein A (RPA) complex, essential for DNA replication, recombination, and repair in eukaryotic cells. RPA 70 kDa subunit is primarily located in the nucleus, where binding to single-stranded DNA (ssDNA) occurs during DNA damage response and replication processes. Nuclear localization of RPA 70 kDa subunit enables immediate access to ssDNA regions requiring repair or replication, facilitating recruitment of other repair proteins and ensuring genomic stability. RPA 70 kDa subunit′s interaction with various cellular and viral proteins coordinates multiple pathways involved in DNA metabolism. RPA 70 kDa subunit′s structure includes a DNA binding domain composed of two homologous subdomains, crucial for forming stable complexes with ssDNA, thus preventing secondary structure formation that could impede replication and repair processes.

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RPA 70 kDa subunit Antibody (B-6) References:

1. Characterization of genetic interactions with RFA1: the role of RPA in DNA replication and telomere maintenance.  |  Smith, J., et al. 2000. Biochimie. 82: 71-8. PMID: 10717390
2. RPA stabilizes the XPA-damaged DNA complex through protein-protein interaction.  |  Wang, M., et al. 2000. Biochemistry. 39: 6433-9. PMID: 10828957
3. Replication protein a 32 kDa subunit (RPA p32) binds the SH2 domain of STAT3 and regulates its transcriptional activity.  |  Kim, J., et al. 2000. Cell Biol Int. 24: 467-73. PMID: 10875894
4. 5' --> 3' molecular polarity of human replication protein A (hRPA) binding to pseudo-origin DNA substrates.  |  Iftode, C. and Borowiec, JA. 2000. Biochemistry. 39: 11970-81. PMID: 11009611
5. Functional characterization of zinc-finger motif in redox regulation of RPA-ssDNA interaction.  |  You, JS., et al. 2000. Biochemistry. 39: 12953-8. PMID: 11041860
6. Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPA.  |  Mer, G., et al. 2000. Cell. 103: 449-56. PMID: 11081631
7. Regulatory functions of the N-terminal domain of the 70-kDa subunit of replication protein A (RPA).  |  Binz, SK. and Wold, MS. 2008. J Biol Chem. 283: 21559-70. PMID: 18515800
8. Characterization of a cDNA encoding the 70-kDa single-stranded DNA-binding subunit of human replication protein A and the role of the protein in DNA replication.  |  Erdile, LF., et al. 1991. J Biol Chem. 266: 12090-8. PMID: 2050703
9. The primary structure of the 32-kDa subunit of human replication protein A.  |  Erdile, LF., et al. 1990. J Biol Chem. 265: 3177-82. PMID: 2406247
10. Rpa4, a homolog of the 34-kilodalton subunit of the replication protein A complex.  |  Keshav, KF., et al. 1995. Mol Cell Biol. 15: 3119-28. PMID: 7760808
11. Structural analysis of human replication protein A. Mapping functional domains of the 70-kDa subunit.  |  Gomes, XV. and Wold, MS. 1995. J Biol Chem. 270: 4534-43. PMID: 7876222
12. Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA.  |  Bochkarev, A., et al. 1997. Nature. 385: 176-81. PMID: 8990123

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RPA 70 kDa subunit Antibody (B-6) has 26 citations in a variety of scientific publications.

Primary antibodies like Anti-RPA 70 kDa subunit Antibody (B-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RPA 70 kDa subunit Antibody (B-6) Specifications

Alternate Product Name: RPA 70 kDa subunit

Product Clone Name: B-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 317-616

Epitope Target: RPA 70 kDa subunit

Epitope Species: human

Product Reactivity/Detection: RPA 70 kDa subunit

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 26 citations in various scientific publications

Available Conjugates/Forms for RPA 70 kDa subunit Antibody (B-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent