RPA 70 kDa subunit Antibody (B-10): sc-166023
- RPA 70 kDa subunit Antibody (B-10) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 268-304 within an internal region of RPA1 of human origin
- recommended for detection of RPA 70 kDa subunit of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and bovine
- TransCruz reagent for ChIP application (sc-166023 X, 200 µg/0.1 ml)
- See RPA 70 kDa subunit (H-7): sc-48425 for RPA 70 kDa subunit antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for RPA 70 kDa subunit Antibody (B-10) for WB applications. These reagents are now offered in bundles with RPA 70 kDa subunit Antibody (B-10) (see ordering information below).
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RPA 70 kDa subunit Antibody (B-10) is a mouse monoclonal IgG1 antibody that detects RPA 70 kDa subunit in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-RPA 70 kDa subunit antibody (B-10) targets a crucial component of the replication protein A complex, which is essential for maintaining genomic stability. RPA 70 kDa subunit (B-10) antibody recognizes a protein that plays a pivotal role in DNA replication, recombination, and repair, acting as a single-stranded DNA-binding protein that stabilizes unwound DNA during these processes. RPA 70 kDa subunit (B-10) monoclonal antibody detects a protein particularly important due to its ability to bind to single-stranded DNA with high affinity, facilitating the recruitment of other proteins involved in DNA damage response and repair mechanisms. Anti-RPA 70 kDa subunit antibody (B-10) targets a protein whose unique structure includes a central DNA-binding domain composed of two homologous subdomains, which enhances interaction with ssDNA and contributes to the formation of a stable RPA-ssDNA complex. A conserved four cysteine-type zinc-finger motif within RPA 70 kDa subunit is critical for modulating RPA-ssDNA interaction in a redox-dependent manner, underscoring the importance of post-translational modifications in regulating function. RPA 70 kDa subunit′s interactions with various cellular and viral proteins further highlight significance in cellular processes, making RPA 70 kDa subunit (B-10) monoclonal antibody an invaluable tool for researchers studying DNA dynamics and repair mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RPA 70 kDa subunit Antibody (B-10) References:
- Replication protein A interactions with DNA. III. Molecular basis of recognition of damaged DNA. | Lao, Y., et al. 2000. Biochemistry. 39: 850-9. PMID: 10653628
- Interaction of DNA polymerase alpha-primase with cellular replication protein A and SV40 T antigen. | Dornreiter, I., et al. 1992. EMBO J. 11: 769-76. PMID: 1311258
- Analysis of the unwinding activity of the dimeric RECQ1 helicase in the presence of human replication protein A. | Cui, S., et al. 2004. Nucleic Acids Res. 32: 2158-70. PMID: 15096578
- DNA replication defects, spontaneous DNA damage, and ATM-dependent checkpoint activation in replication protein A-deficient cells. | Dodson, GE., et al. 2004. J Biol Chem. 279: 34010-4. PMID: 15197179
- Theoretical prediction of the binding free energy for mutants of replication protein A. | Carra, C., et al. 2012. J Mol Model. 18: 3035-49. PMID: 22160652
- Human DNA helicase B (HDHB) binds to replication protein A and facilitates cellular recovery from replication stress. | Guler, GD., et al. 2012. J Biol Chem. 287: 6469-81. PMID: 22194613
- Discovery of a potent stapled helix peptide that binds to the 70N domain of replication protein A. | Frank, AO., et al. 2014. J Med Chem. 57: 2455-61. PMID: 24491171
- Purification and characterization of replication protein A, a cellular protein required for in vitro replication of simian virus 40 DNA. | Wold, MS. and Kelly, T. 1988. Proc Natl Acad Sci U S A. 85: 2523-7. PMID: 2833742
- Isolation and characterization of replication protein A (RP-A) from tobacco cells. | Garcia-Maya, MM. and Buck, KW. 1997. FEBS Lett. 413: 181-4. PMID: 9287140
- The 32- and 14-kilodalton subunits of replication protein A are responsible for species-specific interactions with single-stranded DNA. | Sibenaller, ZA., et al. 1998. Biochemistry. 37: 12496-506. PMID: 9730822
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RPA 70 kDa subunit Antibody (B-10) | sc-166023 | 200 µg/ml | $322.00 | |||
RPA 70 kDa subunit Antibody (B-10): m-IgG Fc BP-HRP Bundle | sc-539990 | 200 µg Ab; 10 µg BP | $361.00 | |||
RPA 70 kDa subunit Antibody (B-10): m-IgG1 BP-HRP Bundle | sc-541832 | 200 µg Ab; 20 µg BP | $361.00 | |||
RPA 70 kDa subunit (B-10) Neutralizing Peptide | sc-166023 P | 100 µg/0.5 ml | $69.00 | |||
RPA 70 kDa subunit Antibody (B-10) X | sc-166023 X | 200 µg/0.1 ml | $322.00 |